LINE-1 retrotransposition events affect endothelial proliferation and migration
| Autor: | Jessica Hauschild, Gyde Steffen, Ferya Banaz-Yaşar, Birthe M. Bongartz, Gerald G. Schumann, Süleyman Ergün |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Histology Endothelium Angiogenesis Somatic cell Swine Medizin Biology Vasculogenesis Testicular Neoplasms Cell Movement medicine Animals Humans Molecular Biology Cell Proliferation Neovascularization Pathologic Prostatic Neoplasms RNA-Directed DNA Polymerase Cell Biology Cell cycle Endonucleases Molecular biology Receptor TIE-2 Cell biology Vascular endothelial growth factor B Endothelial stem cell Medical Laboratory Technology Vascular endothelial growth factor A medicine.anatomical_structure Ki-67 Antigen Long Interspersed Nucleotide Elements Urinary Bladder Neoplasms Endothelium Vascular |
| Zdroj: | Histochemistry and cell biology. 134(6) |
| ISSN: | 1432-119X |
| Popis: | Long interspersed nuclear element-1 (LINE-1, L1) is a retrotransposon which affects the human genome by a variety of mechanisms. While LINE-1 expression is suppressed in the most somatic human cells, LINE-1 elements are activated in human cancer. Recently, high accumulation of LINE-1-encoded ORF1p and ORF2p in endothelial cells of mature human blood vessels was described. Here, we demonstrate that LINE-1 de novo retrotransposition events lead to a reduction of endothelial cell proliferation and migration in a porcine aortic endothelial (PAE) cell model. Cell cycle studies show a G0/G1 arrest in PAE cells harboring LINE-1 de novo retrotransposition events. Remarkably, in in situ analysis LINE-1-encoded ORF2p was not detectable in tumor blood vessels of different human organs while vascular endothelial cells of corresponding normal organs strongly expressed LINE-1 ORF2p. Quantitative RT-PCR analysis revealed that LINE-1 de novo retrotransposition influences selectively the expression of some angiogenic factors such as VEGF and Tie-2. Thus, our data suggest that LINE-1 de novo retrotransposition events might suppress angiogenesis and tumor vascularisation by reducing the angiogenic capacity of vascular endothelial cells. |
| Databáze: | OpenAIRE |
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