Identification of a novel class of genomic DNA-binding sites suggests a mechanism for selectivity in target gene activation by the tumor suppressor protein p53
| Autor: | Kathy Zhao, Lois Resnick-Silverman, Selvon St. Clair, James J. Manfredi, Matthew Maurer |
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| Rok vydání: | 1998 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Transcriptional Activation Response element Cell Cycle Proteins Spodoptera Biology Cell Line Research Communication chemistry.chemical_compound Cyclins Tumor Cells Cultured Genetics Animals Humans cdc25 Phosphatases Binding site Promoter Regions Genetic Gene Cyclin Binding Sites Kinase Antibodies Monoclonal DNA Molecular biology genomic DNA chemistry Cell culture Tumor Suppressor Protein p53 Developmental Biology |
| Zdroj: | Genes & Development. 12:2102-2107 |
| ISSN: | 1549-5477 0890-9369 |
| DOI: | 10.1101/gad.12.14.2102 |
| Popis: | There are two response elements for p53 in the promoter of the gene for the cyclin-dependent kinase inhibitor p21. The binding of p53 to the 5′ site was enhanced by incubation with monoclonal antibody 421, whereas the binding of p53 to the 3′ site was inhibited. Mutational analysis showed that a single-base change caused one element to behave like the other. A response element in the humancdc25C promoter is bound by p53 with properties similar to the 3′ site. These results identify two classes of p53-binding sites and suggest a mechanism for target gene selectivity by p53. |
| Databáze: | OpenAIRE |
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