Oligonucleotide Microchip for the Identification of Infectious Agents of Reproductive System with Simultaneous Analysis of Determinants of Resistance to Antimicrobial Substances
| Autor: | E. I. Dement’eva, B. L. Shaskol’skii, A. V. Chestkov, D. A. Gryadunov, Alexey A. Kubanov, Arvo Leinsoo, M. V. Shpilevaya, Dmitry Deryabin, O. A. Obraztsova |
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| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial 0301 basic medicine 030106 microbiology Microbial Sensitivity Tests urologic and male genital diseases medicine.disease_cause Ureaplasma General Biochemistry Genetics and Molecular Biology Microbiology Gonorrhea 03 medical and health sciences 23S ribosomal RNA Drug Resistance Bacterial Microchip Analytical Procedures medicine Humans Syphilis Treponema pallidum Gene Treponema biology Ureaplasma Infections Nucleic Acid Hybridization General Medicine Mycoplasma biology.organism_classification Antimicrobial Virology Neisseria gonorrhoeae female genital diseases and pregnancy complications Anti-Bacterial Agents Molecular Diagnostic Techniques DNA microarray |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 164:54-60 |
| ISSN: | 1573-8221 0007-4888 |
| Popis: | We developed a multiplexed DNA microarray-based assay allowing identification of 12 causative agents of reproductive tract infections with the simultaneous detection of 47 genetic determinants of resistance to antimicrobial substances. The microarray was tested on 93 isolates of Neisseria gonorrhoeae, 32 isolates of Treponema pallidum and 29 samples of Ureaplasma spp./Mycoplasma spp. The N. gonorrhoeae isolates had multiple mutations in the penA, ponA, rpsJ, gyrA, parC, and mtrR genes; their prognostic value significantly increased when combinations of mutations were detected. In the analyzed T. pallidum isolates, single A2058G substitution in the 23S rRNA gene responsible for macrolide resistance was found. DNA sequences of Ureaplasma spp./Mycoplasma spp. were determined as wild type, which was not fully consistent with the results of analysis of their antimicrobial susceptibility. |
| Databáze: | OpenAIRE |
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