HPLC-DAD phenolic profile, cytotoxic and anti-kinetoplastidae activity of Melissa officinalis

Autor: Luiz Marivando Barros, Maria Celeste Vega Gomez, Emily Pansera Waczuc, Francisco Assis Bezerra da Cunha, Antonia Eliene Duarte, Celestina E. Sobral-Souza, Margareth Linde Athayde, Fernando Gomes Figueredo, Jean Paul Kamdem, Jeferson Luis Franco, Aline Augusti Boligon, Cathia Coronel, Mirian Rolon, Nadghia Figueiredo Leite, Henrique Douglas Melo Coutinho, Samuel V. Brito, Saulo R. Tintino
Rok vydání: 2016
Předmět:
0301 basic medicine
Serial dilution
medicine.drug_class
Cell Survival
Trypanosoma cruzi
Antiprotozoal Agents
Pharmaceutical Science
Context (language use)
01 natural sciences
Melissa
Leishmania braziliensis
Cell Line
Lethal Dose 50
03 medical and health sciences
chemistry.chemical_compound
Rutin
Parasitic Sensitivity Tests
Phenols
Drug Discovery
Caffeic acid
medicine
Kinetoplastida
Leishmania infantum
Chromatography
High Pressure Liquid
Pharmacology
Plants
Medicinal
biology
Traditional medicine
Dose-Response Relationship
Drug
010405 organic chemistry
Plant Extracts
General Medicine
Fibroblasts
biology.organism_classification
0104 chemical sciences
Plant Leaves
030104 developmental biology
Complementary and alternative medicine
chemistry
Biochemistry
Spectrophotometry
Officinalis
Antiprotozoal
Molecular Medicine
Melissa officinalis
Phytotherapy
Zdroj: Pharmaceutical biology. 54(9)
ISSN: 1744-5116
Popis: Context Melissa officinalis subsp. inodora Bornm. (Lamiaceae) has been used since ancient times in folk medicine against various diseases, but it has not been investigated against protozoa. Objective To evaluate the activities of M. officinalis against Leishmania braziliensis, Leishmania infantum and Trypanosoma cruzi as well as its cytotoxicity in fibroblast cell line. Materials and methods The fresh leaves were chopped into 1 cm(2) pieces, washed and macerated with 99.9% of ethanol for 72 h at room temperature. Antiparasitic activity of M. officinalis was accessed by direct counting of cells after serial dilution, while the cytotoxicity of M. officinalis was evaluated in fibroblast cell line (NCTC929) by measuring the reduction of resazurin. The test duration was 24 h. High-performance liquid chromatography (HPLC) was used to characterise the extract. Results The extract at concentrations of 250 and 125 μg/mL inhibited 80.39 and 54.27% of promastigote (LC50 value = 105.78 μg/mL) form of L. infantum, 80.59 and 68.61% of L. brasiliensis (LC50 value = 110.69 μg/mL) and against epimastigote (LC50 value = 245.23 μg/mL) forms of T. cruzi with an inhibition of 54.45 and 22.26%, respectively, was observed. The maximum toxicity was noted at 500 μg/mL with 95.41% (LC50 value = 141.01 μg/mL). The HPLC analysis identified caffeic acid and rutin as the major compounds. Discussion The inhibition of the parasites is considered clinically relevant (
Databáze: OpenAIRE
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