The NIT1 Promoter Allows Inducible and Reversible Silencing of Centrin in Chlamydomonas reinhardtii
| Autor: | Karl-Ferdinand Lechtreck, Bettina Koblenz |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
biology
Inverted repeat Chlamydomonas reinhardtii Articles General Medicine biology.organism_classification Microbiology Molecular biology Blot Aminohydrolases RNA interference Trimethoprim Sulfamethoxazole Drug Combination Centrin Animals Gene silencing RNA Interference Gene Silencing Northern blot Promoter Regions Genetic Molecular Biology Gene |
| Zdroj: | Eukaryotic Cell. 4:1959-1962 |
| ISSN: | 1535-9786 1535-9778 |
| DOI: | 10.1128/ec.4.11.1959-1962.2005 |
| Popis: | An inverted repeat corresponding to parts of the centrin gene of Chlamydomonas reinhardtii was placed downstream of the NIT1 promoter, which is induced by ammonium starvation. After induction, transformants developed centrin deficiency as assayed by immunofluorescence, Western blotting, and Northern blotting. The effect was reversible, demonstrating that the NIT1 promoter allowed controlled RNA interference in Chlamydomonas reinhardtii . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|