Mechanisms by which in vitro meiotic arrest and sexual maturity improve developmental potential of mouse oocytes
| Autor: | Shu-Fen Ning, Jing-He Tan, Xue Sun, Bin Xiao, Juan Lin, Hui-Li Wang, Fei Chen, Shuai Zhu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cytoplasm Transcription Genetic lcsh:Medicine Embryonic Development Article Mice 03 medical and health sciences 0302 clinical medicine Prophase Meiosis Cyclin-dependent kinase Transcription (biology) Roscovitine medicine Animals Sexual Maturation lcsh:Science Gene Alpha-Amanitin Sulfonamides Cumulus Cells 030219 obstetrics & reproductive medicine Multidisciplinary biology Kinase Cyclin-dependent kinase 5 lcsh:R Cyclin-Dependent Kinase 5 Cell Cycle Checkpoints Isoquinolines Oocyte Chromatin Cell biology 030104 developmental biology medicine.anatomical_structure Bucladesine Oocytes biology.protein RNA lcsh:Q Female RNA Polymerase II Cell Nucleolus |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-16119-5 |
| Popis: | To study the relationship between chromatin condensation, gene transcription and developmental competence during oocyte maturation and to explore the mechanisms by which meiotic arrest maintenance (MAM) and sexual maturity improve oocyte competence, we examined effects of MAM with roscovitine or db-cAMP on chromatin condensation, gene transcription and developmental potential of NSN or SN oocytes from prepubertal or adult mice. MAM with roscovitine improved the developmental competence and global gene transcription of prepubertal NSN (prep-NSN) and adult-SN oocytes while having no effect on those of prep-SN oocytes. MAM with db-cAMP facilitated neither development nor transcription in any type of oocytes. MAM with either roscovitine or db-cAMP promoted chromatin condensation of prep-NSN oocytes. MAM with roscovitine promoted gene transcription and chromatin condensation simultaneously through inhibiting cyclin-dependent kinase (CDK) 5 and 2, respectively. The results suggested that MAM with roscovitine improved oocyte competence by promoting gene transcription via inhibiting CDK5. Oocyte cytoplasmic maturation is correlated with gene transcription but not with chromatin condensation. The difference in developmental competence between prepubertal NSN and SN oocytes and between prepubertal and adult SN oocytes was because while the former had not, the latter had completed or acquired the ability for transcription of important genes. |
| Databáze: | OpenAIRE |
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