Bovine Herpesvirus 5 Glycoprotein E Is Important for Neuroinvasiveness and Neurovirulence in the Olfactory Pathway of the Rabbit
| Autor: | Bong Joo Lee, Shafiqul I. Chowdhury, M. L. Weiss, Aykut Ozkul |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Olfactory system
animal diseases viruses Molecular Sequence Data Immunology Central nervous system Virulence Alphaherpesvirinae Microbiology Cell Line law.invention Diagnosis Differential Open Reading Frames Central Nervous System Infections Viral Envelope Proteins law Viral entry Virology medicine Animals Amino Acid Sequence Antigens Viral Cerebral Cortex Gene Rearrangement Recombination Genetic biology virus diseases Herpesviridae Infections Olfactory Pathways Gene rearrangement biochemical phenomena metabolism and nutrition biology.organism_classification Immunohistochemistry Recombinant Proteins Disease Models Animal medicine.anatomical_structure Bovine herpesvirus 5 Insect Science Mutation Recombinant DNA Pathogenesis and Immunity Cattle Rabbits Gene Deletion |
| Zdroj: | Journal of Virology. 74:2094-2106 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.74.5.2094-2106.2000 |
| Popis: | Glycoprotein E (gE) is important for full virulence potential of the alphaherpesviruses in both natural and laboratory hosts. The gE sequence of the neurovirulent bovine herpesvirus 5 (BHV-5) was determined and compared with that of the nonneurovirulent BHV-1. Alignment of the predicted amino acid sequences of BHV-1 and BHV-5 gE open reading frames showed that they had 72% identity and 77% similarity. To determine the role of gE in the differential neuropathogenesis of BHV-1 and BHV-5, we have constructed BHV-1 and BHV-5 recombinants: gE-deleted BHV-5 (BHV-5gEΔ), BHV-5 expressing BHV-1 gE (BHV-5gE1), and BHV-1 expressing BHV-5 gE (BHV-1gE5). Neurovirulence properties of these recombinant viruses were analyzed using a rabbit seizure model (S. I. Chowdhury et al., J. Comp. Pathol. 117:295–310, 1997) that distinguished wild-type BHV-1 and -5 based on their differential neuropathogenesis. Intranasal inoculation of BHV-5 gEΔ and BHV-5gE1 produced significantly reduced neurological signs that affected only 10% of the infected rabbits. The recombinant BHV-1gE5 did not invade the central nervous system (CNS). Virus isolation and immunohistochemistry data suggest that these recombinants replicate and spread significantly less efficiently in the brain than BHV-5 gE revertant or wild-type BHV-5, which produced severe neurological signs in 70 to 80% rabbits. Taken together, the results of neurological signs, brain lesions, virus isolation, and immunohistochemistry indicate that BHV-5 gE is important for efficient neural spread and neurovirulence within the CNS and could not be replaced by BHV-1 gE. However, BHV-5 gE is not required for initial viral entry into olfactory pathway. |
| Databáze: | OpenAIRE |
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