Characterization of the Antinociceptive Activity from Stevia serrata Cav
| Autor: | Max Samuel Mérida-Reyes, Daniel Luiz Reis Simas, Juan Francisco Pérez-Sabino, Manuel Alejandro Muñoz-Wug, Antonio Jorge Ribeiro da Silva, Bessie Evelyn Oliva-Hernández, Thais Biondino Sardella Giorno, Millena Santos Cordeiro, Patricia Dias Fernandes |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Medicine (miscellaneous) (+)-Naloxone Pharmacology Article essential oil General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Oral administration Stevia serrata medicine pain lcsh:QH301-705.5 antinociception Chemistry Atropine 030104 developmental biology Nociception lcsh:Biology (General) Mechanism of action Opioid inflammation Capsaicin medicine.symptom Licking 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Biomedicines, Vol 8, Iss 79, p 79 (2020) Biomedicines Volume 8 Issue 4 |
| ISSN: | 2227-9059 |
| DOI: | 10.3390/biomedicines8040079 |
| Popis: | Background: Stevia serrata Cav. (Asteraceae), widely found in Guatemala, is used to treat gastrointestinal problems. The aim of this study was to demonstrate the antinociceptive and anti-inflammatory effects of the essential oil (EO) and the mechanism of action. Methods: EO was tested in chemical (capsaicin- and glutamate-induced licking response) or thermal (hot plate) models of nociception at 10, 30 or 100 mg/kg doses. The mechanism of action was evaluated using two receptor antagonists (naloxone, atropine) and an enzyme inhibitor (L-NAME). The anti-hyperalgesic effect was evaluated using carrageenan-induced nociception and evaluated in the hot plate. Results: All three doses of EO reduced licking response induced by glutamate, and higher doses reduced capsaicin-induced licking. EO also increased area under the curve, similar to the morphine-treated group. The antinociceptive effect induced by EO was reversed by pretreatment of mice with naloxone (1 mg/kg, ip), atropine (1 mg/kg, ip) or L-NAME (3 mg/kg, ip). EO also demonstrated an anti-hyperalgesic effect. The 100 mg/kg dose increased the latency time, even at 1 h after oral administration and this effect has been maintained until the 96th hour, post-administration. Conclusions: Our data suggest that essential oil of S. serrata presents an antinociceptive effect mediated, at least in part, through activation of opioid, cholinergic and nitrergic pathways. |
| Databáze: | OpenAIRE |
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