Investigation of unmatured hematopoietic cell migration in a mouse model
| Autor: | Aida Vaitkuvienė, Gene Biziulevičienė, Vytautas Kašėta, Giedrė Ramanauskaitė |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
education.field_of_study
Pathology medicine.medical_specialty General Immunology and Microbiology Hematopoietic cell QH301-705.5 General Neuroscience Cell Population mouse contact hypersensitivity model Spleen Biology General Biochemistry Genetics and Molecular Biology Haematopoiesis medicine.anatomical_structure In vivo Immunology medicine Fluorescence microscope Bone marrow Biology (General) General Agricultural and Biological Sciences education hematopoietic lin− cell migration |
| Zdroj: | Open Life Sciences, Vol 5, Iss 5, Pp 585-589 (2010) |
| ISSN: | 2391-5412 |
| Popis: | The objective of this work was to examine the migration of transplanted bone marrow hematopoietic lin− cell population using the BALB/c mouse contact hypersensitivity model in vivo and to determine the time point at which they reach the site of injury (paw edema) as well as other undamaged organs, such as liver and spleen. Female BALB/c mice with induced contact hypersensitivity reaction were intravenously injected with 1×106 cells/mouse lin− cells, labeled with PKH67. The presence of lin− stained cells in mouse tissue sections was evaluated by fluorescent microscopy. After one hour, the labeled cells were found in mice paw edema and liver, and after 4 hours in spleen tissue. Migrated hematopoietic lin− cells remained in liver tissue for 48 h, and in spleen and paw edema at least for 72 h. Migrated stained cells in untreated paw were not found. The results prove that bone marrow unmatured hematopoietic cells are first found in paw edema, where they participate in the inhibition of tissue inflammation; these cells subsequently migrate to the liver and are found in the spleen shortly afterwards. |
| Databáze: | OpenAIRE |
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