Microfluidic extraction and digital quantification of circulating cell-free DNA from serum
| Autor: | Iago Pereiro, Charlotte Proudhon, Sonia Garrigou, Karla Perez-Toralla, Jean-Louis Viovy, Valérie Taly, Stéphanie Descroix, François-Clément Bidard, Fahima Di Federico |
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| Přispěvatelé: | Médecine Personnalisée, Pharmacogénomique, Optimisation Thérapeutique (MEPPOT - U1147), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU), Laboratoire des biomarqueurs tumoraux circulants [Institut Curie, Paris] (SiRIC - Département de recherche translationnelle), Institut Curie [Paris]-Université Paris sciences et lettres (PSL), Institut Curie [Paris], Physico-Chimie-Curie (PCC), Institut Curie-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Université de recherche Paris-Sciences-et-Lettres (Université de recherche PSL)-Institut Curie [Paris], Institut Curie |
| Rok vydání: | 2019 |
| Předmět: |
Chromatography
Chemistry [SDV]Life Sciences [q-bio] Extraction (chemistry) Microfluidics Metals and Alloys 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences DNA extraction Circulating Cell-Free DNA 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound Materials Chemistry Nucleic acid Digital polymerase chain reaction Multiplex Electrical and Electronic Engineering 0210 nano-technology Instrumentation DNA |
| Zdroj: | Sensors and Actuators B: Chemical Sensors and Actuators B: Chemical, Elsevier, 2019, 286, pp.533-539. ⟨10.1016/j.snb.2019.01.159⟩ |
| ISSN: | 0925-4005 |
| Popis: | International audience; Miniaturized devices for the extraction of DNA have been used for assessing genetic material in biological, forensic and environmental samples. However, the ability to isolate trace amounts of highly fragmented DNA from biological fluids remains a challenge. The current work reports a microfluidic approach that combines on line a dynamic magnetic extraction procedure with droplet-based digital PCR (ddPCR). This strategy maximizes the surface area for DNA binding within the chip, in order to capture short DNA fragments, with the possibility of recovering the purified samples into picoliter volumes for high sensitivity mutation detection. The application of this technology to capture circulating cell-free DNA (ccfDNA) from serum samples of cancer patients is demonstrated herein, with efficiencies comparable to standard column-based DNA extraction methods. This technology uses lesser amounts of required material and reagents, and has a higher potential for automation and multiplex DNA analysis. Furthermore, this approach can also be extended for the detection of other circulating biomarkers, such as nucleic acid sequences with aberrant methylation patterns or miRNA. |
| Databáze: | OpenAIRE |
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