Prevention of pacemaker infections with perioperative antimicrobial treatment: an in vitro study
| Autor: | Christian Kuehn, Meike Stiesch, Theodosios Bisdas, Georg Marsch, Ludmilla Knigina, Bakr Mashaqi, Karin Burgwitz, Axel Haverich |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Pacemaker
Artificial Staphylococcus aureus Time Factors medicine.drug_class Cell Survival Myoblasts Skeletal Antibiotics medicine.disease_cause Bacterial Adhesion Microbiology chemistry.chemical_compound Bacitracin Antiseptic Coated Materials Biocompatible Staphylococcus epidermidis Physiology (medical) medicine Escherichia coli Humans Cells Cultured biology business.industry Endothelial Cells Neomycin Equipment Design Fibroblasts biology.organism_classification Antimicrobial Anti-Bacterial Agents chemistry Pseudomonas aeruginosa Anti-Infective Agents Local Vancomycin Equipment Contamination Daptomycin Cardiology and Cardiovascular Medicine business medicine.drug Octenidine dihydrochloride |
| Zdroj: | Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology. 16(4) |
| ISSN: | 1532-2092 |
| Popis: | Aims The antimicrobial treatment of pacemaker casings with antiseptics (povidone-iodine or octenidine dihydrochloride) or antibiotics (vancomycin, daptomycin, cefuroxime, Tazobac, or nebacetin) was analysed in vitro for its biocompatibility and efficacy in preventing the bacterial adhesion of Staphylococcus epidermidis , Staphylococcus aureus , Pseudomonas aeruginosa , and Escherichia coli to cardiac-implantable electrophysiological devices (CIEDs). Methods and results Titan platelets (0.4 cm²) cut from pacemaker casings were impregnated with seven different antimicrobial solutions: two antiseptics and five antibiotics. Subsequently, they were challenged with bacterial contamination by four test strains over a 24 h incubation period. Bacterial adherence was quantified using the colony-forming-unit method after cell recovery with sonication and examined with confocal laser scanning electron microscopy. Simultaneously, the biocompatibility of the antimicrobial impregnation was assessed using pre-treated titan platelets in a culture of human fibroblasts, skeletal myoblasts, and microvascular endothelial cells. After a 48 h incubation, cell vitality was measured using the 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H/tetrazolium monosodium (WST-8) assay. The immersion of pacemaker casings in antiseptic or antibiotic solutions applies an antimicrobial coating that can significantly reduce bacterial adhesion. The studied impregnations differed in their antimicrobial efficacy and toxicity. Conclusion Compared with the two antiseptics and the other tested antibiotics, nebacetin showed the best ratio of efficacy to toxicity. Nebacetin showed good in vitro antibacterial activity against both Gram-positive and Gram-negative pathogens without impairing human cell vitality. It is a safe and effective candidate for CIED impregnation. |
| Databáze: | OpenAIRE |
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