Aqueous Humor Proteomic Alterations Associated with Visual Field Index Parameters in Glaucoma Patients: A Pilot Study
| Autor: | Amy Estes, Shruti Sharma, Sai Karthik Kodeboyina, Kathryn E. Bollinger, David Bogorad, Lane Ulrich, Tae Jin Lee, Ashok Sharma, Wenbo Zhi |
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| Rok vydání: | 2021 |
| Předmět: |
visual field
Apolipoprotein D medicine.medical_specialty genetic structures lcsh:Medicine Glaucoma Aqueous humor Proteomics Article 03 medical and health sciences proteomics 0302 clinical medicine Pattern standard deviation Ophthalmology medicine mass spectrometry 030304 developmental biology 0303 health sciences business.industry lcsh:R C4A General Medicine medicine.disease eye diseases Complement system Visual field glaucoma 030221 ophthalmology & optometry sense organs aqueous humor business |
| Zdroj: | Journal of Clinical Medicine Volume 10 Issue 6 Journal of Clinical Medicine, Vol 10, Iss 1180, p 1180 (2021) |
| ISSN: | 2077-0383 |
| Popis: | Purpose: The purpose of this study was to discover the aqueous humor proteomic changes associated with visual field indices in glaucoma patients. Methods: Aqueous humor samples were analyzed using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). The visual fields were analyzed with the Humphrey Visual Field analyzer. Statistical analyses were performed to discover the relationship between the aqueous humor proteins and visual field parameters including Pattern Standard Deviation (PSD), Visual Field Index (VFI), Mean Deviation (MD) and Glaucoma Hemifield Test (GHT). Results: In total, 222 proteins were identified in 49 aqueous humor samples. A total of 11, 9, 7, and 6 proteins were significantly correlated with PSD, VFI, MD, and GHT respectively. These proteins include apolipoprotein D, members of complement pathway (C1S, C4A, C4B, C8B, and CD14), and immunoglobulin family (IKHV3-9, IGKV2-28). Conclusion: Several proteins involved in immune responses (immunoglobulins and complement factors) and neurodegeneration (apolipoprotein D) were identified to be associated with abnormal visual field parameters. These findings provide targets for future studies investigating precise molecular mechanisms and new therapies for glaucomatous optic neuropathy. |
| Databáze: | OpenAIRE |
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