Extracellular calcium is required for the maintenance of plasma membrane integrity in nucleated cells
| Autor: | Aksentsev Sl, Sergei V. Kotelevtsev, Sergei N. Orlov |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Erythrocytes Physiology Biology Hemolysis Muscle Smooth Vascular Cell membrane Hemoglobins chemistry.chemical_compound BAPTA Nucleated cell medicine Extracellular Animals Humans Magnesium Rats Wistar Egtazic Acid Molecular Biology Cell Nucleus Calcium metabolism L-Lactate Dehydrogenase Cell Membrane Sodium Fishes Cell Biology Haemolysis Rats EGTA medicine.anatomical_structure chemistry Biochemistry Biophysics Calcium Intracellular HeLa Cells |
| Zdroj: | Cell Calcium. 38:53-57 |
| ISSN: | 0143-4160 |
| DOI: | 10.1016/j.ceca.2005.03.006 |
| Popis: | In contrast to rat and human erythrocytes, nucleated erythrocytes from two fish species (Cyprinus carpio and Salmo trutta) underwent almost complete haemolysis in 20 min of EDTA addition. Using Ca2+/Mg2+ EGTA-citrate buffer, we observed that half-maximal haemolysis of fish erythrocytes occurs at [Ca2+]o approximately 10 microM independently of extracellular Mg2+ concentration. Attenuation of [Ca2+]o with EGTA also decreased stability of the plasma membrane of vascular smooth muscle cells (VSMC) and HeLa cells, indicated by a three- to five-fold elevation of lactate dehydrogenase release and passive permeability of plasma membrane for Na+. In VSMC, EGTA lowered [Ca2+]i by approximately 20%. This effect was absent in VSMC-loaded with the intracellular Ca2+ chelator BAPTA. In contrast to EGTA, BAPTA did not affect haemoglobin release from fish erythrocytes and passive permeability for Na+ in VSMC. Viewed collectively, our data show that in nucleated cells, extracellular Ca2+ plays a crucial role in the maintenance of plasma membrane integrity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect