A comparison of glucose concentration in paired specimens collected in serum separator and fluoride/potassium oxalate blood collection tubes under survey ‘field’ conditions
| Autor: | Sherry L. Perkins, Peter W. F. Fischer, Lois Fernandez, Stephen P. J. Brooks, Penny Jee, Mari-Jill Klein |
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| Rok vydání: | 2013 |
| Předmět: |
Blood Glucose
Blood Specimen Collection Chromatography Potassium oxalate Chemistry Oxalic Acid Clinical Biochemistry Analytical chemistry Anticoagulants General Medicine medicine.disease Hemolysis chemistry.chemical_compound Reducing Agents medicine Humans Sodium Fluoride National level Blood Collection Tube Specimen processing Fluoride Blood drawing Field conditions |
| Zdroj: | Clinical Biochemistry. 46:285-288 |
| ISSN: | 0009-9120 |
| Popis: | Objectives There are no direct comparisons of blood glucose values in samples collected with barrier serum tubes (SST™) and NaF/potassium oxalate (NaF/KOx) plasma tubes. Collection of samples in SST™ tubes can offer considerable savings and specimen processing advantages for national level surveys. Design and methods Serum and plasma samples were collected under ‘field conditions’ from a single draw of 3692 individuals participating in the Canadian Health Measures Survey. The samples were analyzed retrospectively using the VITROS GLU Slide method (glucose oxidase-based). Results There was a high rate of hemolysis in the NaF/KOx tubes (86.2%) while hemolysis was infrequently observed with the SST™ tubes (2%). Comparing only blood draws where no hemolysis was observed in both tubes (n = 495; paired t -test) showed no effect of tube type on serum/plasma glucose concentrations. This was also observed when data was restricted to cases when only SST™ samples were not hemolyzed (n = 3546; paired t -test). Conclusions These data show that both collection tubes can be used under survey collection and processing conditions to measure glucose with our assay system with no difference in reported results. |
| Databáze: | OpenAIRE |
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