Introduction to approaches and tools for the evaluation of protein cysteine oxidation
Autor: | Cristina M. Furdui, S. Bruce King, Leslie B. Poole |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Cell
Chemical biology Oxidative phosphorylation Computational biology 010402 general chemistry Proteomics 01 natural sciences Biochemistry Redox Article 03 medical and health sciences medicine Humans Cysteine Molecular Biology Cellular proteins 030304 developmental biology Fluorescent Dyes chemistry.chemical_classification 0303 health sciences Reactive oxygen species Chemistry Proteins 0104 chemical sciences medicine.anatomical_structure Molecular Probes Electrophoresis Polyacrylamide Gel Oxidation-Reduction Protein Processing Post-Translational |
Zdroj: | Essays Biochem |
Popis: | Oxidative modifications of cysteine thiols in cellular proteins are pivotal to the way signal-stimulated reactive oxygen species are sensed and elicit appropriate or sometimes pathological responses, but the dynamic and often transitory nature of these modifications offer a challenge to the investigator trying to identify such sites and the responses they elicit. A number of reagents and workflows have been developed to identify proteins undergoing oxidation and to query the timing, extent and location of such modifications, as described in this minireview. While no approach is perfect to capture all the redox information in a functioning cell, best practices described herein can enable considerable insights into the “redox world” of cells and organisms. |
Databáze: | OpenAIRE |
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