Differential expression of coxsackievirus and adenovirus receptor in endomyocardial tissue of patients with myocarditis
| Autor: | Adda J. García‑Chequer, Cesar A. Flores‑García, Luis A. Moreno‑Ruiz, Miguel Cruz, Gustavo E. García‑Becerril, Martín H. Garrido‑Garduño, Carmen Maldonado‑Bernal, Miguel A. Ares, María G. Cárdenas‑Mondragón, Luz M. Gómez‑Jiménez, Aníbal E. Cruz‑Montalvo |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Male Cancer Research medicine.medical_specialty Coxsackie and Adenovirus Receptor-Like Membrane Protein Myocarditis Heart disease Down-Regulation Coxsackievirus Biochemistry Gastroenterology 03 medical and health sciences Young Adult 0302 clinical medicine Internal medicine Genetics medicine Humans RNA Messenger Young adult Molecular Biology myocardial inflammation biology Oncogene business.industry Myocardium Retrospective cohort study Articles Middle Aged medicine.disease biology.organism_classification Molecular medicine Pathophysiology coxsackie virus and adenovirus receptor expression 030104 developmental biology Cross-Sectional Studies Oncology 030220 oncology & carcinogenesis Molecular Medicine Female business |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 |
| Popis: | Recent studies demonstrated that the expression of coxsackievirus and adenovirus receptor (CAR) is implicated in the pathophysiology of myocarditis. The aim of the present study was to assess the association between active and borderline myocarditis and CAR expression in endomyocardial tissues, and analyze the association between CAR expression and treatment response. An analytic, cross‑sectional, retrospective study was performed in 26 patients with myocarditis and 10 control subjects without heart disease. Myocardial biopsies were obtained and CAR transcription was measured by reverse transcription‑quantitative polymerase chain reaction analysis. The association between CAR mRNA levels and the response to immunosuppressive or conventional therapy (treatment responders, n=17; non‑responders, n=9) or with the type of histological myocarditis (active myocarditis, n=16; borderline myocarditis, n=10) was analyzed. CAR transcription levels were significantly lower (P=0.012) in patients with myocarditis compared with controls, and a significant decrease was observed (P=0.023) in CAR mRNA levels among patients with borderline myocarditis compared with the no myocarditis group. Patients responding to therapy exhibited higher CAR mRNA levels (P=0.036) compared with patients not responding to treatment, as evaluated based on clinical and echocardiographic criteria (immunosuppressive therapy, n=8; conventional therapy, n=1). Myocarditis in non‑responders was associated with fewer clinical manifestations and lower CAR mRNA levels. A significant difference was only found regarding the use of oral steroids in patients with active myocarditis who responded to treatment (P=0.02), with no difference in borderline myocarditis. In conclusion, the transcriptional level of CAR is low in the endomyocardial tissue of patients with myocarditis, and it is lower in borderline myocarditis and in non‑responder patients. These findings may enable early identification of patients who may benefit from treatment and timely determination of prognosis. |
| Databáze: | OpenAIRE |
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