Galactosylated liposome as a dendritic cell-targeted mucosal vaccine for inducing protective anti-tumor immunity
| Autor: | Hung Jun Lin, Der Zen Liu, Shen Fu Lin, Pi-Hui Liang, Hsiao Wen Wang, Yi-You Huang, Wen Yu Tsai, Mei Yin Chien, Ping Lun Jiang |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Ovalbumin
medicine.medical_treatment Antigen delivery Biomedical Engineering Galactosylated liposome Cancer immunotherapy Mucosal vaccine Cancer Vaccines Biochemistry Biomaterials Mice In vivo Immunity medicine Animals Molecular Biology Administration Intranasal Liposome biology Chemistry Galactose General Medicine Dendritic cell Dendritic Cells Neoplasms Experimental Dendritic cell targeted Mice Inbred C57BL carbohydrates (lipids) Nasal Mucosa Treatment Outcome Immunology Liposomes biology.protein Nasal administration lipids (amino acids peptides and proteins) Antibody Biotechnology |
| Zdroj: | Acta Biomaterialia. :356-367 |
| ISSN: | 1742-7061 |
| DOI: | 10.1016/j.actbio.2014.09.019 |
| Popis: | Mucosal surfaces contain specialized dendritic cells (DCs) that are able to recognize foreign pathogens and mount protective immunity. We previously demonstrated that intranasal administration of targeted galactosylated liposomes can elicit mucosal and systemic antibody responses. In the present study, we assessed whether galactosylated liposomes could act as an effective DC-targeted mucosal vaccine that would be capable of inducing systemic anti-tumor immunity as well as antibody responses. We show that targeted galactosylated liposomes effectively facilitated antigen uptake by DCs beyond that mediated by unmodified liposomes both in vitro and in vivo. Targeted galactosylated liposomes induced higher levels of pro-inflammatory cytokines than unmodified liposomes in vitro. C57BL/6 mice thrice immunized intranasally with ovalbumin (OVA)-encapsulated galactosylated liposomes produced high levels of OVA-specific IgG antibodies in their serum. Spleen cells from mice receiving galactosylated liposomes were restimulated with OVA and showed significantly augmented levels of IFN-γ, IL-4, IL-5 and IL-6. In addition, intranasal administration of OVA-encapsulated beta-galactosylated liposomes resulted in complete protection against EG7 tumor challenge in C57BL/6 mice. Taken together, these results indicate that nasal administration of a galactosylated liposome vaccine mediates the development of an effective immunity against tumors and might be useful for further clinical anti-tumoral applications. |
| Databáze: | OpenAIRE |
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