Antimicrobial peptide induced colloidal transformations in bacteria-mimetic vesicles : combining in silico tools and experimental methods
| Autor: | Linda Sandblad, Madeleine Ramstedt, Stefan Salentinig, Yeny Pillco-Valencia, Rafael V.M. Freire, Thereza A. Soares, Gabriel C. A. da Hora |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pore Forming Cytotoxic Proteins
medicine.medical_treatment Antimicrobial peptides Lipid Bilayers Biophysics POPG 02 engineering and technology Molecular Dynamics Simulation 010402 general chemistry 01 natural sciences Physical Chemistry Cathelicidin Cryo-TEM Biomaterials Colloid and Surface Chemistry Dynamic light scattering Anti-Infective Agents Annan medicinsk grundvetenskap Teoretisk kemi medicine Humans Vesicles Theoretical Chemistry Other Basic Medicine Phospholipids Fysikalisk kemi biology Bacteria Chemistry Vesicle LL-37 Self-assembly SAXS 021001 nanoscience & nanotechnology biology.organism_classification Antimicrobial Condensed Matter Physics Biofysik 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Membrane Membrane curvature 0210 nano-technology Den kondenserade materiens fysik Coarse-grain molecular dynamics simulations |
| Popis: | With the growing challenges of bacteria becoming resistant to conventional antibiotics, antimicrobial peptides (AMPs) may offer a potential alternative. One of the most studied AMPs, the human cathelicidin derived AMP LL-37 is notable for its antimicrobial activity even though its mechanism of action is not fully understood yet. This work investigates the interaction of LL-37 with 1-Palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-(1-glycerol) (POPG) vesicles, which were employed as a bacterial membrane model given the common presence of this phospholipid in the bacterial membrane. Experimental techniques including small angle X-ray scattering, transmission electron microscopy and dynamic light scattering were used to characterize the interactions among LL-37 and POPG. Molecular dynamics simulations complement the experimental studies with molecular-level insights into the process. LL-37 was discovered to actively and critically interact with the POPG vesicles, modifying the membrane curvature that eventually leads to structural transformations from vesicles to mixed micelles. The results shed light on the mechanisms underlying the interactions among LL-37 and bacteria mimetic vesicles and can guide the further development of AMP based antimicrobial materials and therapies. |
| Databáze: | OpenAIRE |
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