Fluorescence aptameric sensor for isothermal circular strand-displacement polymerization amplification detection of adenosine triphosphate
| Autor: | Shusheng Zhang, Xuxu Xie, Qiao Zhang, Weiling Song |
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| Rok vydání: | 2014 |
| Předmět: |
Aptamer
Biomedical Engineering Biophysics Biosensing Techniques Fluorescence Polymerization chemistry.chemical_compound Adenosine Triphosphate Limit of Detection Electrochemistry Humans Polymerase Detection limit biology General Medicine Aptamers Nucleotide Spectrometry Fluorescence chemistry Biochemistry Nucleic acid biology.protein Primer (molecular biology) Adenosine triphosphate DNA Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 61 |
| ISSN: | 1873-4235 |
| Popis: | In this work, isothermal circular strand-displacement polymerization amplification assay is developed for highly specific and sensitive detection of adenosine triphosphate (ATP). The amplification process consists of circular common target molecule-displacement polymerization (CCDP) and circular nucleic acid strand-displacement polymerization (CNDP). In the presence of ATP, the complementary strand was released from the aptamer by the target recognition of ATP, and catalyzed the subsequent cycle reaction. With the polymerase and primer, the displaced target triggers the process of CCDP. With the involvement of nicking endonuclease, the released complementary strand triggers the CNDP. Combined CCDP with CNDP, the exponentially produced fluorescence probes are obtained, achieving a detection limit of ATP as low as 2.6 × 10(-10)M. Moreover, the proposed strategy exhibits an excellent specificity and is successfully applied in real sample assay which demonstrates potential application in practical samples. |
| Databáze: | OpenAIRE |
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