In vivo immunomodulatory effects of adipose-derived mesenchymal stem cells conditioned medium in experimental autoimmune encephalomyelitis
| Autor: | Sara Soudi, Forouzan Yousefi, Masoud Soleimani, Seyed Mahmoud Hashemi, Massoumeh Ebtekar |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Encephalomyelitis Autoimmune Experimental Multiple Sclerosis medicine.medical_treatment Immunology Population Adipose tissue Pharmacology Lymphocyte Activation T-Lymphocytes Regulatory Immunomodulation 03 medical and health sciences Mice Cell Movement medicine Splenocyte Immunology and Allergy Animals Humans IL-2 receptor education Cells Cultured Cell Proliferation education.field_of_study business.industry Experimental autoimmune encephalomyelitis Mesenchymal stem cell FOXP3 Brain Mesenchymal Stem Cells Stem-cell therapy medicine.disease Mice Inbred C57BL Disease Models Animal 030104 developmental biology Adipose Tissue Culture Media Conditioned Cytokines business |
| Zdroj: | Immunology letters. 172 |
| ISSN: | 1879-0542 |
| Popis: | Mesenchymal stem cells (MSCs) are well known to possess neuroprotective and immunomodulatory effects, due to cell-to-cell interaction and their soluble factors. We conducted a comparative analysis of the immunomodulatory properties of adipose tissue mesenchymal stem cells (AT-MSCs) and their conditioned media (CM), derived from C57/BL6 mice, for mitigating the adverse clinical course of experimental autoimmune encephalomyelitis (EAE). We measure IL4, IL17 and IFNɣ production of supernatant from spleen cells. We analyzed brain cell infiltration, splenocyte proliferation and evaluated the percentage of CD4+CD25+FOXP3+splenic cell population in all EAE C57/BL6 mice. AT-MSCs and its conditioned medium induced CD4+CD25+FOXP3+regulatory T cells after in vitro co-culture with naive T cells. There is no significant difference in the clinical scores and body weight of EAE mice treated with AT-MSCs and CM. The reduction in proliferative responses and brain cell infiltration was more pronounced in mice injected with CM than other groups. It is found that the percentage of splenic CD4+CD25+FOXP3+ population as well as the level of IL4 production in mice administrated with AT-MSCs is increased compared to other animals. Our results suggest that AT-MSCs-derived CM is promising in stem cell therapy, due to their neuroprotective and immunomudulatory properties. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect