Extrinsic intestinal denervation modulates tumor development in the small intestine of ApcMin/+ mice
| Autor: | Hendrik Seeliger, Marlon R. Schneider, Martin E. Kreis, Petra Benhaqi, Alexandra Dietrich, Michael Schemann, Verena Liu, Michael S. Kasparek |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Pathology Colorectal cancer Adenomatous Polyposis Coli Protein Ileum Biology 600 Technik Medizin angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten Vagus nerve Mice Internal medicine Intestine Small medicine Animals Intestinal Cancer Visceral innervation Denervation Mice Knockout Tyrosine hydroxylase medicine.disease Small intestine Tumor Burden ddc Intestinal cancer Disease Models Animal medicine.anatomical_structure Endocrinology Cell Transformation Neoplastic Oncology Duodenum Colorectal Neoplasms Research Article |
| Zdroj: | Journal of Experimental & Clinical Cancer Research : CR |
| Popis: | Background Innervation interacts with enteric immune responses. Chronic intestinal inflammation is associated with increased risk of colorectal cancer. We aimed to study potential extrinsic neuronal modulation of intestinal tumor development in a mouse model. Methods Experiments were performed with male ApcMin/+ or wild type mice (4 weeks old, body weight approximately 20 g). Subgroups with subdiaphragmatic vagotomy (apcV/wtV), sympathetic denervation of the small intestine (apcS/wtS) or sham operated controls (apcC/wtC) were investigated (n = 6-14 per group). Three months after surgical manipulation, 10 cm of terminal ileum were excised, fixed for 48 h in 4% paraformaldehyde and all tumors were counted and their area determined in mm2 (mean ± standard error of the mean (SEM)). Whole mounts of the muscularis of terminal ileum and duodenum (internal positive control) were also stained for tyrosine hydroxylase to confirm successful sympathetic denervation. Results Tumor count in ApcMin/+ mice was 62 ± 8 (apcC), 46 ± 11 (apcV) and 54 ± 8 (apcS) which was increased compared to wildtype controls with 4 ± 0.5 (wtC), 5 ± 0.5 (wtV) and 5 ± 0.6 (wtS; all p |
| Databáze: | OpenAIRE |
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