A cohort study of cucumber greenhouse workers' exposure to microorganisms as measured using NGS and MALDI-TOF MS and biomarkers of systemic inflammation
| Autor: | Amal Markouch, Jesper Bælum, Ulla Vogel, John Kerr White, Trine Thilsing, Anne Mette Madsen, Kira Tendal, Vinni Mona Hansen, Jeppe Lund Nielsen, Sarah Kadhim, Nadieh de Jonge |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbacterium
Air Microbiology Air Pollutants Occupational 010501 environmental sciences 01 natural sciences Biochemistry Microbiology Cohort Studies 03 medical and health sciences 0302 clinical medicine Ralstonia Ascomycota Occupational Exposure Humans 030212 general & internal medicine Serum amyloid A 0105 earth and related environmental sciences General Environmental Science Podosphaera fusca Inflammation biology Pseudomonas Fungi High-Throughput Nucleotide Sequencing Biodiversity Acinetobacter Occupational exposure biology.organism_classification Stenotrophomonas maltophilia Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Cucumis sativus Fungal exposure Biomarkers Cladosporium Environmental Monitoring Acute-phase response |
| Zdroj: | Madsen, A M, White, J K, Markouch, A, Kadhim, S, de Jonge, N, Thilsing, T, Hansen, V M, Bælum, J, Nielsen, J L, Vogel, U & Tendal, K 2021, ' A cohort study of cucumber greenhouse workers' exposure to microorganisms as measured using NGS and MALDI-TOF MS and biomarkers of systemic inflammation ', Environmental Research, vol. 192, 110325 . https://doi.org/10.1016/j.envres.2020.110325 Madsen, A M, White, J K, Markouch, A, Kadhim, S, Jonge, N D, Thilsing, T, Hansen, V M, Bælum, J, Nielsen, J L, Vogel, U & Tendal, K 2021, ' A cohort study of cucumber greenhouse workers’ exposure to microorganisms as measured using NGS and MALDI-TOF MS and biomarkers of systemic inflammation ', Environmental Research, vol. 192, 110325 . https://doi.org/10.1016/j.envres.2020.110325 |
| DOI: | 10.1016/j.envres.2020.110325 |
| Popis: | Work in greenhouses entails exposure to airborne fungi and bacteria. The aims of this study are to obtain knowledge about whether exposure to fungal and bacterial genera and species during work in a cucumber greenhouse is affected by work tasks, and whether a cohort of greenhouse workers' serum levels of serum amyloid A (SAA) and C-reactive protein (CRP), biomarkers of systemic inflammation, are associated with this. Data on personal exposure to airborne fungal and bacterial species measured over 4 years as well as serum levels of SAA and CRP sampled over two years were analyzed. For data analysis, the main work tasks were grouped into three different groups, called ‘grouped work task’. Microorganisms were identified using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF MS) and next-generation sequencing (NGS). The ‘daily exposure’ of greenhouse workers' were as follows: 4.8 × 10 4 CFU bacteria/m 3, 1.4 × 10 6 CFU fungi/m 3, and 392 EU/m 3 of endotoxin. Workers were exposed to many different microbial species including several species within the genera Acinetobacter, Bacillus, Microbacterium, Pseudomonas, Staphylococcus, and Streptomyces. The genera Ralstonia and Cladosporium were found in most samples. The exposure levels as well as the microbial composition were associated significantly with grouped work task and season with high exposures during tasks in close contact with mature and old plants and in the autumn. CRP and SAA levels were also associated with exposure level and grouped work tasks. The Shannon-Wiener indices were not different in the 3 ‘grouped work tasks’. Several specific species including e.g. Halomonas elongata, Stenotrophomonas maltophilia, Podosphaera fusca, and Wallemia spp. were found frequently or in high concentrations in the exposures associated with the highest levels of CRP and SAA. The microorganisms S. maltophilia, P. fusca, and Wallemia spp. were also found on the cucumber plant leaves. In conclusion, both exposure level and the species composition seem to have an effect on the serum levels of CRP and SAA of exposed workers. The greenhouse workers were exposed to only a few species characterized as human pathogens. |
| Databáze: | OpenAIRE |
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