Intronic regulatory elements determine the divergent expression patterns of AGAMOUS-LIKE6 subfamily members in Arabidopsis
| Autor: | Arturo Bolaños, Ueli Grossniklaus, Ramarmurthy Baskar, Mark D. Curtis, Philipp M. Schlüter, Jacqueline Gheyselinck, Stephen E. Schauer |
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| Přispěvatelé: | University of Zurich, Grossniklaus, U |
| Rok vydání: | 2009 |
| Předmět: |
0106 biological sciences
Subfamily Arabidopsis thaliana Arabidopsis Plant Science 580 Plants (Botany) 01 natural sciences Conserved sequence 1307 Cell Biology 10126 Department of Plant and Microbial Biology plant gene Gene Expression Regulation Plant Genes Reporter 1110 Plant Science MADS domain protein Promoter Regions Genetic Conserved Sequence Phylogeny Genetics 0303 health sciences biology Agamous gene expression regulation reporter gene Enhancer Elements Genetic Regulatory sequence enhancer region intron Molecular Sequence Data MADS Domain Proteins Genes Plant 03 medical and health sciences promoter region 1311 Genetics Silencer Elements Transcriptional Enhancer 030304 developmental biology Arabidopsis protein Arabidopsis Proteins Intron nucleotide sequence Cell Biology biology.organism_classification Introns molecular genetics silencer element Brassicaceae Subfunctionalization metabolism 010606 plant biology & botany |
| Zdroj: | The Plant journal : for cell and molecular biology |
| ISSN: | 1365-313X |
| Popis: | Summary The screening of enhancer detector lines in Arabidopsis thaliana has identified genes that are specifically expressed in the sporophytic tissue of the ovule. One such gene is the MADS-domain transcription factor AGAMOUS-LIKE6 (AGL6), which is expressed asymmetrically in the endothelial layer of the ovule, adjacent to the developing haploid female gametophyte. Transcription of AGL6 is regulated at multiple stages of development by enhancer and silencer elements located in both the upstream regulatory region and the large first intron. These include a bipartite enhancer, which requires elements in both the upstream regulatory region and the first intron, active in the endothelium. Transcription of the AGL13 locus, which encodes the other member of the AGL6 subfamily in Arabidopsis, is also regulated by elements located in the upstream regulatory region and in the first intron. There is, however, no overlapping expression of AGL6 and AGL13 except in the chalaza of the developing ovule, as was shown using a dual gene reporter system. Phylogenetic shadowing of the first intron of AGL6 and AGL13 homologs from other Brassicaceae identified four regions of conservation that probably contain the binding sites of transcriptional regulators, three of which are conserved outside Brassicaceae. Further phylogenetic analysis using the protein-encoding domains of AGL6 and AGL13 revealed that the MADS DNA-binding domain shows considerable divergence. Together, these results suggest that AGL6 and AGL13 show signs of subfunctionalization, with divergent expression patterns, regulatory sequences and possibly functions. Journal compilation � 2009 Blackwell Publishing Ltd. |
| Databáze: | OpenAIRE |
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