Antihyperglycemic and hypoglycemic activities of the aqueous leaf extract of Rubus Erlangeri Engl (Rosacea) in mice
| Autor: | Akeberegn Gorems Ayele, Ephrem Engidawork, Prem Kumar |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
BGL
blood glucose level Antioxidant Physiology FBG Fasting blood glucose medicine.medical_treatment QD415-436 Pharmacology Hypoglycemia Biochemistry Rubus erlangeri Diabetes mellitus In vitro In vivo TFC Total flavonoid content GAE Gallic acid equivalent weight medicine QP1-981 OGTT Oral glucose tolerance IC50 NO nitric oxide Streptozotocin business.industry DM Diabetes mellitus STZ Streptozotocin General Medicine medicine.disease Acute toxicity Original Research Paper DNS Dinitrosalicylic acid DPPH diphenyl-1-picrylhydrazine IP intraperitoneal business medicine.drug |
| Zdroj: | Metabolism Open Metabolism Open, Vol 11, Iss, Pp 100118-(2021) |
| ISSN: | 2589-9368 |
| DOI: | 10.1016/j.metop.2021.100118 |
| Popis: | Background: The prevalence of diabetes mellitus is on the inexorable rise despite the promises of a wide range of conventional medications. Thus, there is a need to scientifically investigate plants for antidiabetic effect. Methods: After the Rubus Erlanrige Engl (Rosaceae) leaf has been decocted, the plant extract's antidiabetic activity was first investigated in vitro and then in vivo. The in vitro activity was assessed using 3, 5-Dinitrosalicylic acid, and 2,2-diphenyl-1-picrylhydrazine method for α-amylase inhibition and antioxidant effect respectively. On the other hand, the in vivo antidiabetic activity was carried out in normoglycemic, glucose loaded (2.5 g/kg) and single dose streptozotocin (200 mg/kg) induced diabetic mice. Results: Acute toxicity study showed the extract is safe with ≥2 g/kg. The in vitro results demonstrated the extract has an IC50 of 7.34 ± 0.02 and 10.38 ± 0.0.62 μg/ml for antioxidant and α-amylase inhibition activity respectively. On the other hand, the in vivo study revealed that the extract significantly reduced blood glucose level following glucose loading. The extract did not, however, produce a significant reduction of glucose level in normal mice indicating low risk of hypoglycemia. The extract also significantly decreased blood glucose levels in streptozotocin-induced diabetic mice. In the single dose study, the extract lowered blood glucose level all except by lower dose at the 3rd and 4th h (p |
| Databáze: | OpenAIRE |
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