Direct detection of Vibrio cholerae in stool samples
| Autor: | R. A. Ugalde, Norma Binsztein, Isabel Chinen, G. D. Pollevick, Marta Rivas, P. Varela, A. C. C. Frasch |
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| Rok vydání: | 1994 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Cholera Toxin Molecular Sequence Data Argentina Enzyme-Linked Immunosorbent Assay medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity law.invention Microbiology Feces Cholera law Vibrionaceae medicine Humans Base sequence Vibrio cholerae Polymerase chain reaction DNA Primers Base Sequence biology medicine.disease biology.organism_classification Evaluation Studies as Topic Genes Bacterial Bacteria Research Article |
| Zdroj: | Journal of Clinical Microbiology. 32:1246-1248 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.32.5.1246-1248.1994 |
| Popis: | A direct method to detect Vibrio cholerae in stool samples was developed by using a PCR procedure that did not require a DNA purification step. Dilution (1/100) of stool samples prevented inhibition of the reaction by contaminants, and two consecutive PCRs, the second one with a nested primer, achieved the desired sensitivity. Comparison of the results obtained from stool swab samples processed by the two-step PCR and by an enzyme-linked immunosorbent assay using GM1 as the capture molecule showed that the former is more sensitive and gave positive results even when V. cholerae was not culturable or dead. |
| Databáze: | OpenAIRE |
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