Developing Bottom-Up Induced Pluripotent Stem Cell Derived Solid Tumor Models Using Precision Genome Editing Technologies
| Autor: | Kelsie L. Becklin, Garrett M. Draper, Rebecca A. Madden, Mitchell G. Kluesner, Tomoyuki Koga, Miller Huang, William A. Weiss, Logan G. Spector, David A. Largaespada, Branden S. Moriarity, Beau R. Webber |
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| Rok vydání: | 2022 |
| Předmět: |
Gene Editing
Stem Cell Research - Induced Pluripotent Stem Cell Stem Cell Research - Induced Pluripotent Stem Cell - Human Induced Pluripotent Stem Cells Human Genome Review Article Stem Cell Research Regenerative Medicine Neoplasms Genetics Humans CRISPR-Cas Systems Stem Cell Research - Embryonic - Human Cancer Biotechnology |
| Zdroj: | The CRISPR journal, vol 5, iss 4 CRISPR J |
| ISSN: | 2573-1602 2573-1599 |
| DOI: | 10.1089/crispr.2022.0032 |
| Popis: | Advances in genome and tissue engineering have spurred significant progress and opportunity for innovation in cancer modeling. Human induced pluripotent stem cells (iPSCs) are an established and powerful tool to study cellular processes in the context of disease-specific genetic backgrounds; however, their application to cancer has been limited by the resistance of many transformed cells to undergo successful reprogramming. Here, we review the status of human iPSC modeling of solid tumors in the context of genetic engineering, including how base and prime editing can be incorporated into "bottom-up" cancer modeling, a term we coined for iPSC-based cancer models using genetic engineering to induce transformation. This approach circumvents the need to reprogram cancer cells while allowing for dissection of the genetic mechanisms underlying transformation, progression, and metastasis with a high degree of precision and control. We also discuss the strengths and limitations of respective engineering approaches and outline experimental considerations for establishing future models. |
| Databáze: | OpenAIRE |
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