Scalable Microgravity Simulator Used for Long-Term Musculoskeletal Cells and Tissue Engineering
| Autor: | Monica Zocchi, Fabian Ille, Carsten Haack, Simon L. Wuest, Christina Giger-Lange, Jeanette A.M. Maier, Alessandra Cazzaniga, Marcel Egli, Adrian Koller, Sara Castiglioni |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
Cell Culture Techniques Human bone Chemical interaction bone marrow stem cells Catalysis Article Inorganic Chemistry lcsh:Chemistry Laboratory flask Mice Bioreactors Tissue engineering Osteogenesis Animals Humans New device Physical and Theoretical Chemistry Muscle Skeletal Molecular Biology lcsh:QH301-705.5 Spectroscopy Simulation Weightlessness Simulation simulated microgravity Tissue Engineering Weightlessness Organic Chemistry myoblasts Cell Differentiation Mesenchymal Stem Cells General Medicine Computer Science Applications Osteogenic medium Simulated microgravity lcsh:Biology (General) lcsh:QD1-999 |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 23 International Journal of Molecular Sciences, Vol 21, Iss 8908, p 8908 (2020) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms21238908 |
| Popis: | We introduce a new benchtop microgravity simulator (MGS) that is scalable and easy to use. Its working principle is similar to that of random positioning machines (RPM), commonly used in research laboratories and regarded as one of the gold standards for simulating microgravity. The improvement of the MGS concerns mainly the algorithms controlling the movements of the samples and the design that, for the first time, guarantees equal treatment of all the culture flasks undergoing simulated microgravity. Qualification and validation tests of the new device were conducted with human bone marrow stem cells (bMSC) and mouse skeletal muscle myoblasts (C2C12). bMSC were cultured for 4 days on the MGS and the RPM in parallel. In the presence of osteogenic medium, an overexpression of osteogenic markers was detected in the samples from both devices. Similarly, C2C12 cells were maintained for 4 days on the MGS and the rotating wall vessel (RWV) device, another widely used microgravity simulator. Significant downregulation of myogenesis markers was observed in gravitationally unloaded cells. Therefore, similar results can be obtained regardless of the used simulated microgravity devices, namely MGS, RPM, or RWV. The newly developed MGS device thus offers easy and reliable long-term cell culture possibilities under simulated microgravity conditions. Currently, upgrades are in progress to allow real-time monitoring of the culture media and liquids exchange while running. This is of particular interest for long-term cultivation, needed for tissue engineering applications. Tissue grown under real or simulated microgravity has specific features, such as growth in three-dimensions (3D). Growth in weightlessness conditions fosters mechanical, structural, and chemical interactions between cells and the extracellular matrix in any direction. |
| Databáze: | OpenAIRE |
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