Arabidopsis RAB8A, RAB8B and RAB8D Proteins Interact with Several RTNLB Proteins and are Involved in the Agrobacterium tumefaciens Infection Process

Autor:	Yin-Tzu Liu, Choun-Sea Lin, Hsin-Nung Chang, Pei-Ru Chien, Shin-Fei Chi, Fan-Chen Huang, Hau-Hsuan Hwang
Rok vydání:	2021
Předmět:	biology Arabidopsis Proteins Physiology Agrobacterium Arabidopsis Membrane Proteins Cell Biology Plant Science General Medicine Agrobacterium tumefaciens biology.organism_classification Cell biology rab1 GTP-Binding Proteins Chloroplast Proteins Bimolecular fluorescence complementation Transformation (genetics) Gene Expression Regulation Plant rab GTP-Binding Proteins Pseudomonas syringae Arabidopsis thaliana Rab Plant Diseases
Zdroj:	Plant and Cell Physiology. 62:1572-1588
ISSN:	1471-9053 0032-0781
DOI:	10.1093/pcp/pcab112
Popis:	Arabidopsis thaliana small GTP-binding proteins, AtRAB8s, associate with the endomembrane system and modulate tubulovesicular trafficking between compartments of the biosynthetic and endocytic pathways. There are five members in Arabidopsis, namely AtRAB8A-8E. Yeast two-hybrid assays, bimolecular fluorescence complementation assays and glutathione-S-transferase pull-down assays showed that RAB8A, 8B and 8D interacted with several membrane-associated reticulon-like (AtRTNLB) proteins in yeast, plant cells and in vitro. Furthermore, RAB8A, 8B and 8D proteins showed interactions with the Agrobacterium tumefaciens virulence protein, VirB2, a component of a type IV secretion system (T4SS). A. tumefaciens uses a T4SS to transfer T-DNA and Virulence proteins to plants, which causes crown gall disease in plants. The Arabidopsis rab8A, rab8B and rab8D single mutants showed decreased levels of Agrobacterium-mediated root and seedling transformation, while the RAB8A, 8B and 8D overexpression transgenic Arabidopsis plants were hypersusceptible to A. tumefaciens and Pseudomonas syringae infections. RAB8A-8E transcripts accumulated differently in roots, rosette leaves, cauline leaves, inflorescence and flowers of wild-type plants. In summary, RAB8A, 8B and 8D interacted with several RTNLB proteins and participated in A. tumefaciens and P. syringae infection processes.
Databáze:	OpenAIRE
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