EphrinB3 restricts endogenous neural stem cell migration after traumatic brain injury
| Autor: | Ross Bullock, Ann M. Turnley, Alisa Turbic, Daniel J. Liebl, Jose Mier, Kirsty J. Dixon, Shyam Gajavelli |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male animal structures Adolescent Rostral migratory stream Traumatic brain injury Subventricular zone Ephrin-B3 Biology Article 03 medical and health sciences Mice 0302 clinical medicine Neuroblast Neural Stem Cells Cell Movement Neuroblast migration Brain Injuries Traumatic medicine Animals Humans Progenitor cell Cell Proliferation Mice Knockout Medicine(all) fungi General Medicine Anatomy Cell Biology Middle Aged medicine.disease Neural stem cell Cortex (botany) Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure nervous system Female Neuroscience 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cell Research. 17(3):504-513 |
| ISSN: | 1873-5061 |
| DOI: | 10.1016/j.scr.2016.09.029 |
| Popis: | Traumatic brain injury (TBI) leads to a series of pathological events that can have profound influences on motor, sensory and cognitive functions. Conversely, TBI can also stimulate neural stem/progenitor cell proliferation leading to increased numbers of neuroblasts migrating outside their restrictive neurogenic zone to areas of damage in support of tissue integrity. Unfortunately, the factors that regulate migration are poorly understood. Here, we examine whether ephrinB3 functions to restrict neuroblasts from migrating outside the subventricular zone (SVZ) and rostral migratory stream (RMS). We have previously shown that ephrinB3 is expressed in tissues surrounding these regions, including the overlying corpus callosum (CC), and is reduced after controlled cortical impact (CCI) injury. Our current study takes advantage of ephrinB3 knockout mice to examine the influences of ephrinB3 on neuroblast migration into CC and cortex tissues after CCI injury. Both injury and/or ephrinB3 deficiency led to increased neuroblast numbers and enhanced migration outside the SVZ/RMS zones. Application of soluble ephrinB3-Fc molecules reduced neuroblast migration into the CC after injury and limited neuroblast chain migration in cultured SVZ explants. Our findings suggest that ephrinB3 expression in tissues surrounding neurogenic regions functions to restrict neuroblast migration outside the RMS by limiting chain migration. |
| Databáze: | OpenAIRE |
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