Structure and Function of an RNA-Reading Thermostable DNA Polymerase
| Autor: | Kay Diederichs, Konrad Bergen, Oliver Nolte, Markus Wieland, Andreas Marx, Nina Blatter, Jutta Mayer, Wolfram Welte |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
DNA polymerase
Base pair DNA polymerase II polymerase chain reaction DNA-Directed DNA Polymerase Polymerase Chain Reaction Catalysis DNA polymerases Humans structural biology Polymerase DNA clamp biology Molecular Structure Chemistry General Chemistry Reverse Transcription reverse transcription Molecular biology enzyme engineering Biochemistry Rolling circle replication ddc:540 biology.protein RNA Primase DNA polymerase I |
| Popis: | Unfaithful: The structure of a DNA-dependent DNA polymerase, whose parental ancestor has no significant reverse transcriptase activity, was obtained for the first time incorporating a nucleotide against an RNA template and then structurally characterized. The DNA polymerase variant has significant reverse transcriptase activity that allows its application in PCR-based molecular diagnostics. |
| Databáze: | OpenAIRE |
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