Germacrone exerts anti-cancer effects on gastric cancer through induction of cell cycle arrest and promotion of apoptosis
| Autor: | Junyong Zhang, Xiangguo Tian, Lei Wu, Lifen Wang, Hua Feng |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cell cycle checkpoint
Blotting Western BGC823 cells Cell Apoptosis Germacrone Cell cycle arrest Sesquiterpenes Germacrane 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Stomach Neoplasms Cell Line Tumor Oils Volatile medicine Humans MTT assay Viability assay Cyclin B1 Cell Proliferation Cell growth Cell Cycle Checkpoints lcsh:Other systems of medicine Flow Cytometry lcsh:RZ201-999 medicine.anatomical_structure Complementary and alternative medicine chemistry 030220 oncology & carcinogenesis Cancer research Gastric cancer 030217 neurology & neurosurgery Research Article |
| Zdroj: | BMC Complementary Medicine and Therapies, Vol 20, Iss 1, Pp 1-9 (2020) BMC Complementary Medicine and Therapies |
| ISSN: | 2662-7671 |
| DOI: | 10.1186/s12906-019-2810-3 |
| Popis: | BackgroundGermacrone is one of the natural bioactive compounds found inRhizoma curcumaessential oils. In this study, the potential anti-cancer effect of germacrone in gastric cancer cell line BGC823 was investigated.MethodsThe cell viability and proliferative activity were assessed, and cell cycle analysis was also performed. Hoechst 33258 and Annexin V/PI double staining was used for detection of cell apoptosis. Protein profiles of cell cycle-related and apoptosis-related proteins were assessed.ResultsMTT assay revealed that germacrone had marked cytotoxicity on BGC823 cells. Germacrone induced cell cycle arrest in the G2/M phase via remarkably decreased expression levels of cyclin B1, cdc 2 and cdc 25c. In addition, the treatment with germacrone induced caspase-3 activity and PARP cleavage. These findings demonstrated the effects of germacrone on inhibiting cell proliferation through induction of G2/M phase cell cycle arrest and promotion of cell apoptosis. It also indicated that germacrone functioned through modulations of cell cycle-associated protein expression and mitochondria-mediated apoptosis.ConclusionThese findings will be valuable as the molecular basis for the germacrone-mediated anti-cancer effect against gastric cancer. |
| Databáze: | OpenAIRE |
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