The effect of Gly–Gln [ß-endorphin30–31] on morphine-evoked serotonin and GABA efflux in the nucleus accumbens of conscious rats
| Autor: | R. Levent Büyükuysal, Sami Aydin, Sinan Cavun, M. Sertac Yilmaz, William R. Millington, Nesrin Filiz Başaran |
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| Přispěvatelé: | Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Farmakoloji Anabilim Dalı., Basaran, Nesrin F., Büyükuysal, R. Levent, Yılmaz, M. Sertaç, Aydın, Sami, Çavun, Sinan, AAC-9702-2019, AAH-1657-2021, AAH-1571-2021 |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Unclassified drug Dopamine efflux Dopamine Microdialysis Reduces ethanol intake Glycyl levo glutamine[beta endorphin 30-31] 5-ht3 receptor antagonists Glycyl-L-glutamine Pharmacology Animal tissue Nucleus Accumbens Rats Sprague-Dawley 0302 clinical medicine Endocrinology Dipeptide 4 aminobutyric acid Dipeptide derivative Dopamine release Serotonin efflux Extracellular space Intracerebroventricular drug administration Beta endorphin derivative gamma-Aminobutyric Acid Endocrinology & metabolism Priority journal Morphine integumentary system Neuromodulation Chemistry Amine transport Glycylglutamine Dipeptides General Medicine Conditioned place preference Beta-endorphin Sprague dawley rat Neurology embryonic structures Drug mechanism medicine.drug Narcotics Serotonin Gaba efflux animal structures Consciousness Sodium chloride Amino acid transport Narcotic agent Opioid Nucleus accumbens Neurosciences & neurology Concentration response Article gamma-Aminobutyric acid Central-nervous-system 03 medical and health sciences Cellular and Molecular Neuroscience Reward 4 aminobutyric acid release Dose response Nucleus accumbens shell medicine Extracellular Animals Opiate antagonist Animal experiment Aging Amoxicillin Contractility Invitro Freely moving rats Injections Intraventricular Serotonin release Drug effects Animal Endocrine and Autonomic Systems Neurosciences Dorsal raphe nucleus Nonhuman 5 7-dihydroxytryptamine lesions Rats Induced dopamine efflux Drug efficacy Metabolism 030104 developmental biology Rat Controlled study Ventral tegmental area 030217 neurology & neurosurgery High performance liquid chromatography |
| Zdroj: | Neuropeptides. 58:23-29 |
| ISSN: | 0143-4179 |
| Popis: | Glycyl-L-glutamine (Gly-Gln; beta-endorphin(30-31)) is an endogenous dipeptide synthesized through the post-translational processing of beta-endorphin(1-31). Central Gly-Gln administration inhibits the rewarding properties of morphine and attenuates morphine tolerance, dependence and withdrawal although it does not interfere with morphine analgesia. In an earlier study, we found that Gly-Gln inhibits morphine-induced dopamine efflux in the nucleus accumbens (NAc), consistent with its ability to inhibit morphine reward. To further investigate the mechanism responsible for its central effects we tested whether i.c.v. Gly-Gln administration influences the rise in extracellular serotonin and GABA concentrations evoked by morphine in the NAc. Conscious rats were treated with Gly-Gln (100 nmol/5 mu l) or saline i.c.v. followed, 2 min later, by morphine (2.5 mg/kg) or saline i.p. and extracellular serotonin and GABA concentrations were analyzed by microdialysis and HPLC. Morphine administration increased extracellular serotonin and GABA concentrations significantly within 20 min, as shown previously. Unexpectedly, Gly-Gln also increased extracellular serotonin concentrations significantly in control animals. Combined treatment with Gly-Gln + morphine also elevated extracellular serotonin concentrations although the magnitude of the response did not differ significantly from the effect of Gly-Gln or morphine, given alone suggesting that Gly-Gin suppressed morphine induced serotonin efflux. Gly-Gln abolished the morphine-induced rise in extracellular GABA concentrations but had no effect on extracellular GABA when given alone to otherwise untreated animals. These data show that Gly-Gln stimulates NAc serotonin efflux and, together with earlier studies, support the hypothesis that Gly-Gln inhibits the rewarding effects of morphine by modulating morphine induced dopamine, GABA and serotonin efflux in the NAc. |
| Databáze: | OpenAIRE |
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