The Internal Conduit System of the Swine Inverted Lymph Node

Autor: Dubreil, Laurence, Ledevin, Mireille, Hervet, Caroline, Menard, Déborah, Philippe, Claire, Michel, François J., Larcher, Thibaut, Meurens, François, Bertho, Nicolas
Přispěvatelé: Expertise en Anatomie Pathologique (APEX), Physiopathologie Animale et bioThérapie du muscle et du système nerveux (PAnTher), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biologie, Epidémiologie et analyse de risque en Santé Animale (BIOEPAR), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Neurobiologie de la Méditerranée [Aix-Marseille Université] (INMED - INSERM U1249), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Saskatchewan [Saskatoon] (U of S), APEX, PAnTher, INRAE, Oniris is supported by Pays de la Loire, IBISA and NeurATRIS translational research infrastructure for innovative therapies in NeuroSciences. Biogenouest (the network of technology core facilities in Western France in life sciences and the environment, and by the Conseil Régional des Pays de la Loire). APEX platform is a Center of Excellence Nikon. Reagents and equipment were purchased thanks to the establishment grant obtained by FM from the Région Pays de la Loire (RFI Food for tomorrow-Cap aliment).
Rok vydání: 2022
Předmět:
Zdroj: Frontiers in Immunology
Frontiers in Immunology, 2022, 13, pp.869384. ⟨10.3389/fimmu.2022.869384⟩
ISSN: 1664-3224
DOI: 10.3389/fimmu.2022.869384
Popis: International audience; Lymph nodes (LN) are the crossroad where naïve lymphocytes, peripheral antigens and antigen presenting cells contact together in order to mount an adaptive immune response. For this purpose, LN are highly organized convergent hubs of blood and lymphatic vessels that, in the case of B lymphocytes, lead to the B cell follicles. Herein take place the selection and maturation of B cell clones producing high affinity antibodies directed against various antigens. Whereas the knowledge on the murine and human LN distribution systems have reached an exquisite precision those last years, the organization of the antigens and cells circulation into the inverted porcine LN remains poorly described. Using up to date microscopy tools, we described the complex interconnections between afferent lymphatics and blood vessels, perifollicular macrophages, follicular B cells and efferent blood vessels. We observed that afferent lymphatic sinuses presented an asymmetric Lyve-1 expression similar to the one observed in murine LN, whereas specialized perifollicular sinuses connect the main afferent lymphatic sinus to the B cell follicles. Finally, whereas it was long though that mature B cells egress from the inverted LN in the T cell zone through HEV, our observations are in agreement with mature B cells accessing the efferent blood circulation in the efferent, subcapsular area. This understanding of the inverted porcine LN circuitry will allow a more accurate exploration of swine pathogens interactions with the immune cells inside the LN structures. Moreover, the mix between similarities and differences of porcine inverted LN circuitry with mouse and human normal LN shall enable to better apprehend the functions and malfunctions of normal LN from a new perspective.
Databáze: OpenAIRE
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