Reduction of myocardial reperfusion injury with human soluble complement receptor type 1 (BRL 55730)
| Autor: | Donald E. Griswold, John W. Egan, L. M. Hillegass, Richard A.G. Smith, Edward F. Smith, Martin J. Hibbs, Robert C. Gagnon |
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| Rok vydání: | 1993 |
| Předmět: |
Male
medicine.medical_specialty Neutrophils Myocardial Infarction Myocardial Reperfusion Injury Complement receptor Rats Sprague-Dawley Pharmacokinetics Internal medicine medicine Animals Humans Myocardial infarction Peroxidase Pharmacology biology business.industry medicine.disease Recombinant Proteins Rats Receptors Complement Disease Models Animal medicine.anatomical_structure Ventricle Myeloperoxidase Anesthesia Circulatory system Cardiology biology.protein business Reperfusion injury Blood vessel |
| Zdroj: | European journal of pharmacology. 236(3) |
| ISSN: | 0014-2999 |
| Popis: | This study was designed to evaluate the cardioprotective effects of a solubilized human complement receptor, sCR1, in the rat subjected to myocardial infarction. Following coronary artery occlusion for 0.5 h and reperfusion for 24 h (MI/R group), myocardial infarct size (determined by planimetric analysis) was 18.3 +/- 2.1% of the left ventricle (n = 16), while myeloperoxidase activity (a biochemical marker of neutrophil activation) was increased from 0.94 +/- 0.09 U/g tissue in the sham occluded + vehicle group to 2.96 +/- 0.17 U/g tissue in the MI/R + vehicle treated group (P0.01). Injection of sCR1 (5 mg/kg i.v., 5 min prior to coronary artery occlusion) produced plasma concentrations of 154 +/- 4 microgram/ml 1 min prior to coronary artery occlusion, and concentrations of 86 +/- 2 and 58 +/- 3 micrograms/ml at 40 min and 125 min after dosing (n = 6). sCR1 reduced myocardial infarct size to 11.3 +/- 2.2% of the left ventricle, and attenuated the increase in myeloperoxidase activity to 2.11 +/- 0.20 U/g tissue (n = 18; P0.01, compared to the MI/R + vehicle group). Administration of sCR1 5 min prior to reperfusion afforded a 25.3% non-significant reduction in myocardial injury. These results suggest a beneficial effect of sCR1 in myocardial ischemia/reperfusion injury by reducing the infiltration of neutrophils and attenuating the extent of myocardial injury. |
| Databáze: | OpenAIRE |
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