The novel adipokine WISP1 associates with insulin resistance and impairs insulin action in human myotubes and mouse hepatocytes
| Autor: | Nicole Seebeck, Tina Hörbelt, Volker Lange, Yves Van Nieuwenhove, Silke Hornemann, Olga Pivovarova, Frederique Van de Velde, Marlies Bekaert, Maria Rödiger, Oliver Kuss, Elisabeth Friedl, Bruno Lapauw, G. Hege Thoresen, Wenke Jonas, Andreas Pfeiffer, Daniella Herzfeld de Wiza, D. Margriet Ouwens, C Tacke, Annette Schürmann, Natalia Rudovich, Mariya Markova, Anke Rosenthal |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Blood Glucose
0301 basic medicine medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Muscle Fibers Skeletal Adipokine Enzyme-Linked Immunosorbent Assay 030209 endocrinology & metabolism FOXO1 Type 2 diabetes Intra-Abdominal Fat CCN Intercellular Signaling Proteins Mice 03 medical and health sciences 0302 clinical medicine Insulin resistance Proto-Oncogene Proteins Internal medicine Internal Medicine medicine Animals Humans Phosphorylation Glycogen synthase biology Adiponectin business.industry Insulin medicine.disease Receptor Insulin Insulin receptor 030104 developmental biology Endocrinology Hepatocytes biology.protein Insulin Resistance business Signal Transduction |
| ISSN: | 0012-186X |
| Popis: | Aims/hypothesis: Wingless-type (Wnt) inducible signalling pathway protein-1 (WISP1) has been recently identified as a proinflammatory adipokine. We examined whether WISP1 expression and circulating levels are altered in type 2 diabetes and whether WISP1 affects insulin signalling in muscle cells and hepatocytes. Methods: Serum and visceral adipose tissue (VAT) biopsies, for analysis of circulating WISP1 levels by ELISA and WISP1 mRNA expression by real-time quantitative RT-PCR, were collected from normal-weight men (control group, n = 33) and obese men with (n = 46) and without type 2 diabetes (n = 56) undergoing surgery. Following incubation of primary human skeletal muscle cells (hSkMCs) and murine AML12 hepatocytes with WISP1 and insulin, insulin signalling was analysed by western blotting. The effect of WISP1 on insulin-stimulated glycogen synthesis and gluconeogenesis was investigated in hSkMCs and murine hepatocytes, respectively. Results: Circulating WISP1 levels were higher in obese men (independent of diabetes status) than in normal-weight men (mean [95% CI]: 70.8 [55.2, 86.4] ng/l vs 42.6 [28.5, 56.6] ng/l, respectively; p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink