Superoxide generation by human monocytes and macrophages
| Autor: | Gerald W. King, Stephen J. Weiss, Albert F. LoBuglio |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Phagocytosis
Monocytes Superoxide dismutase chemistry.chemical_compound Chronic granulomatous disease Superoxides Extracellular medicine Humans Macrophage biology Superoxide Dismutase Superoxide Macrophages Monocyte Zymosan Hematology medicine.disease Molecular biology Oxygen medicine.anatomical_structure chemistry Biochemistry biology.protein |
| Zdroj: | American Journal of Hematology. 4:1-8 |
| ISSN: | 1096-8652 0361-8609 |
| DOI: | 10.1002/ajh.2830040102 |
| Popis: | Intracellular and extracellular superoxide (O2.-) generation by human monocytes and macrophages was quantitated by the nitroblue tetrazolium (NBT) reduction method. Human monocytes reduced 4.4 +/- 0.9 nmoles/10(6) cells/15 minutes with an increase to 12.4 +/- 1.3 during phagocytosis of zymosan. Based on inhibition by superoxide dismutase, superoxide generation of these cells was 1.8 +/- 0.9 nmoles in the resting state and 16.8 +/- 2.8 nmoles with zymosan phagocytosis. Human macrophages obtained by thoracentesis had comparable levels of NBT reduction and O2.-generation. Monocytes from a patient with chronic granulomatous disease demonstrated no increment in O2.-production during phagocytosis. Thus, human monocytes and macrophages appear capable of generating substantial amounts of O2.-during phagocytosis which may play an important role in bactericidal and other cell functions. |
| Databáze: | OpenAIRE |
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