VHSV Single Amino Acid Polymorphisms (SAPs) Associated With Virulence in Rainbow Trout

Autor: Francesco Pascoli, Michele Gastaldelli, Joëlle Cabon, Michel Brémont, Thierry Morin, Yannick Blanchard, David Ryder, Anna Toffan, Anna Luiza Farias Alencar, Valentina Panzarin, Niels Jørgen Olesen, Stéphane Biacchesi, Argelia Cuenca, Carlos P. Dopazo, Miriam Abbadi, Lénaïg Louboutin
Přispěvatelé: Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), DTU Aqua, National Institute of Aquatic Resources, Technical University of Denmark [Lyngby] (DTU), Laboratoire de Ploufragan-Plouzané-Niort [ANSES], Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Centre for Environment, Fisheries and Aquaculture Science [Weymouth] (CEFAS), Instituto de Acuicultura, Universidade de Santiago de Compostela, Virologie et Immunologie Moléculaires (VIM (UR 0892)), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Novimark Project (G88F13000660001) funded by the Animal Health and Welfare (Anihwa) ERA-Net Consortium., UK’s Department for Environment, Food and Rural Affairs (Defra) under contract C7277
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Frontiers in Microbiology
Frontiers in Microbiology, Frontiers Media, 2020, 11, ⟨10.3389/fmicb.2020.01984⟩
Panzarin, V, Cuenca, A, Gastaldelli, M, Alencar, A L F, Pascoli, F, Morin, T, Blanchard, Y, Cabon, J, Louboutin, L, Ryder, D, Abbadi, M, Toffan, A, Dopazo, C P, Biacchesi, S, Brémont, M & Olesen, N J 2020, ' VHSV Single Amino Acid Polymorphisms (SAPs) Associated With Virulence in Rainbow Trout ', Frontiers in Microbiology, vol. 11, 1984 . https://doi.org/10.3389/fmicb.2020.01984
Frontiers in Microbiology, Vol 11 (2020)
ISSN: 1664-302X
DOI: 10.3389/fmicb.2020.01984⟩
Popis: International audience; The Viral Hemorrhagic Septicemia Virus (VHSV) is an OIE notifiable pathogen widespread in the Northern Hemisphere that encompasses four genotypes and nine subtypes. In Europe, subtype Ia impairs predominantly the rainbow trout industry causing severe rates of mortality, while other VHSV genotypes and subtypes affect a number of marine and freshwater species, both farmed and wild. VHSV has repeatedly proved to be able to jump to rainbow trout from the marine reservoir, causing mortality episodes. The molecular mechanisms regulating VHSV virulence and host tropism are not fully understood, mainly due to the scarce availability of complete genome sequences and information on the virulence phenotype. With the scope of identifying in silico molecular markers for VHSV virulence, we generated an extensive dataset of 55 viral genomes and related mortality data obtained from rainbow trout experimental challenges. Using statistical association analyses that combined genetic and mortality data, we found 38 single amino acid polymorphisms scattered throughout the complete coding regions of the viral genome that were putatively involved in virulence of VHSV in trout. Specific amino acid signatures were recognized as being associated with either low or high virulence phenotypes. The phylogenetic analysis of VHSV coding regions supported the evolution toward greater virulence in rainbow trout within subtype Ia, and identified several other subtypes which may be prone to be virulent for this species. This study sheds light on the molecular basis for VHSV virulence, and provides an extensive list of putative virulence markers for their subsequent validation.
Databáze: OpenAIRE
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