The ontogeny of the glucose-6-phosphatase enzyme in human embryonic and fetal red blood cells
| Autor: | Robert Hume, Ann Burchell, Anne Hallas, Sima Pazouki |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Erythrocytes Ontogeny Immunocytochemistry Gestational Age Biology Blood cell Pregnancy Internal medicine medicine Humans Fetus Obstetrics and Gynecology Embryo Embryo Mammalian Fetal Blood Embryonic stem cell Immunohistochemistry Red blood cell medicine.anatomical_structure Endocrinology Pediatrics Perinatology and Child Health Erythrocyte Count Glucose-6-Phosphatase Female |
| Zdroj: | Early human development. 42(2) |
| ISSN: | 0378-3782 |
| Popis: | We have shown for the first time that the microsomal glucose-6-phosphatase enzyme protein is present in human embryonic and fetal red blood cells and the ontogeny of its expression has been determined. In the earliest embryos, red cells are predominantly of the primitive megaloblastic type. Circulating red cells in the primitive megaloblastic series are predominantly nucleated and glucose-6-phosphatase immunopositive. Non-nucleated, immunoreactive megaloblastic cells are in a minority. In fetuses > 12 weeks gestation, the erythrocytes are of the definitive normoblastic series and in the transitional period of switch-over in late embryonic-early fetal life, up to 30% of glucose-6-phosphatase immunopositive cells are definitive normoblastic in type, with a variable contribution from nucleated and non-nucleated cells. Thereafter, the number of immunopositive cells in the definitive normoblastic series decreases such that after 12 weeks gestation it is less than 5%. The fact that a predominantly hepatic protein in adults (glucose-6-phosphatase) is present in embryonic and fetal red blood cells, particularly nucleated red cells, raises the possibility of diagnosis of disorders of liver protein expression in nucleated fetal red cells isolated from the first trimester maternal circulation. |
| Databáze: | OpenAIRE |
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