Effect of arsenite and growth in biofilm conditions on the evolution of Thiomonas sp. CB2

Autor: Freel, Kelle, Fouteau, Stéphanie, Roche, David, Farasin, Julien, Huber, Aline, Koechler, Sandrine, Peres, Martina, Chiboub, Olfa, Cruveiller, Stephane, Varet, Hugo, Proux, Caroline, Deschamps, Julien, Briandet, Romain, Torchet, Rachel, Cruveiller, Stéphane, Lièvremont, Didier, Coppée, Jean-Yves, Barbe, Valérie, Arsène-Ploetze, Florence
Přispěvatelé: Génétique moléculaire, génomique, microbiologie (GMGM), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Génomique métabolique (UMR 8030), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Transcriptome et Epigénome (PF2), Institut Pasteur [Paris], MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), This work was supported by the Université de Strasbourg, the Centre National de la Recherche Scientifique (CNRS) and the Region Alsace (J.F.). This study was also financed by THIOFILM (ANR-12-ADAP-0013) projects. K.C.F., O.C. and J.F. were supported by the Agence Nationale de la Recherche, ANR THIOFILM (ANR-12-ADAP-0013). The Transcriptome and EpiGenome Platform is a member of the France Génomique consortium (ANR10-NBS-09-08), ANR-12-ADAP-0013,THIOFILM,Rôle des biofilms dans l'adaptation et la variabilité génomique des bactéries du genre Thiomonas, impliqués dans les processus de remédiation naturelle dans les drainages miniers :(2012), ANR-10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Strasbourg (UNISTRA)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Microbial Genomics
Microbial Genomics, Society for General Microbiology, 2020, 6 (10), pp.1-18. ⟨10.1099/mgen.0.000447⟩
Microbial Genomics, 2020, 6 (10), pp.1-18. ⟨10.1099/mgen.0.000447⟩
Microbial Genomics, Society for General Microbiology, 2020, 6 (10), ⟨10.1099/mgen.0.000447⟩
ISSN: 2057-5858
Popis: Thiomonasbacteria are ubiquitous at acid mine drainage sites and play key roles in the remediation of water at these locations by oxidizing arsenite to arsenate, favouring the sorption of arsenic by iron oxides and their coprecipitation. Understanding the adaptive capacities of these bacteria is crucial to revealing how they persist and remain active in such extreme conditions. Interestingly, it was previously observed that after exposure to arsenite, when grown in a biofilm, some strains ofThiomonasbacteria develop variants that are more resistant to arsenic. Here, we identified the mechanisms involved in the emergence of such variants in biofilms. We found that the percentage of variants generated increased in the presence of high concentrations of arsenite (5.33 mM), especially in the detached cells after growth under biofilm-forming conditions. Analysis of gene expression in the parent strain CB2 revealed that genes involved in DNA repair were upregulated in the conditions where variants were observed. Finally, we assessed the phenotypes and genomes of the subsequent variants generated to evaluate the number of mutations compared to the parent strain. We determined that multiple point mutations accumulated after exposure to arsenite when cells were grown under biofilm conditions. Some of these mutations were found in what is referred to as ICE19, a genomic island (GI) carrying arsenic-resistance genes, also harbouring characteristics of an integrative and conjugative element (ICE). The mutations likely favoured the excision and duplication of this GI. This research aids in understanding howThiomonasbacteria adapt to highly toxic environments, and, more generally, provides a window to bacterial genome evolution in extreme environments.
Databáze: OpenAIRE
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