Evaluation of hyperbranched polyglycerol for cold perfusion and storage of donor kidneys in a pig model of kidney autotransplantation
| Autor: | Yang Wen, Liang Wang, Zhongli Huang, Irina Cafeeva, Xiaowei Li, Xin Jiang, Jayachandran N. Kizhakkedathu, Caigan Du, Youguang Zhao, Qiunong Guan, Shadan Li, Christopher Nguan, Donald E. Brooks, Hao Luo |
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| Rok vydání: | 2020 |
| Předmět: |
Glycerol
Male medicine.medical_specialty endocrine system hyperbranched polyglycerol Materials science Adenosine Polymers Swine medicine.medical_treatment Allopurinol Organ Preservation Solutions Biomedical Engineering Urology Cold storage Kidney Transplantation Autologous Original Research Report Biomaterials 03 medical and health sciences 0302 clinical medicine Raffinose donor organ storage Original Research Reports medicine Animals Insulin Viaspan colloid Blood urea nitrogen 030304 developmental biology 0303 health sciences organ transplantation Organ Preservation preservation solution Glutathione Kidney Transplantation Autotransplantation Nephrectomy 3. Good health Transplantation Perfusion medicine.anatomical_structure 030220 oncology & carcinogenesis |
| Zdroj: | Journal of Biomedical Materials Research. Part B, Applied Biomaterials |
| ISSN: | 1552-4981 |
| Popis: | Hyperbranched polyglycerol (HPG) is a biocompatible polyether polymer that is a potential colloid component in a preservation solution for suppressing interstitial edema during cold storage of a donor organ. This study evaluated the outcomes of kidney transplants after cold perfusion and storage with a HPG‐based preservation solution (HPGS) in a pig model of kidney autotransplantation. The left kidneys of farm pigs (weighing 35–45 kg) were perfused with and stored in either cold HPGS or standard UW solution (UWS), followed by transplantation to the right side after right nephrectomy. The survival and function of transplants were determined by the urine output, and serum creatinine (SCr) and blood urea nitrogen (BUN) of recipients. Transplant injury was examined by histological analysis. Here, we showed that there was no significant difference between HPGS and UWS in the prevention of tissue edema, but HPGS was more effective than UWS for initial blood washout of kidney perfusion and for the prevention of cold ischemia injury during cold storage. After autotransplantation, the kidneys preserved with HPGS (HPG group) had better functional recovery than those with UWS (UW group), indicated by significantly more urine output and lower levels of SCr and BUN. The survived grafts in HPG group had less tissue damage than those in UW group. In conclusion, as compared to the UWS the HPGS has less negative impact on kidney cold ischemia during cold storage, resulting in improving immediate functional recovery after transplantation, suggesting that HPG is a promising colloid for donor kidney preservation. |
| Databáze: | OpenAIRE |
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