Repeated stress reduces mucociliary clearance in animals with chronic allergic airway inflammation
| Autor: | Ricardo Henrique Marques, Alessandra Choqueta de Toledo, Rafael Almeida-Reis, Edna A. Leick-Maldonado, Fabiana G. Reis, Milton A. Martins, Carla Máximo Prado, Iolanda F.L.C. Tibério, Marisa Dolhnikoff |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine medicine.medical_specialty Hydrocortisone Ovalbumin Physiology Mucociliary clearance medicine.medical_treatment Guinea Pigs Internal medicine Adrenal Glands medicine Animals Cilia Respiratory system Saline Swimming Inflammation Lung biology Inhalation business.industry General Neuroscience Organ Size respiratory system Mucus Disease Models Animal medicine.anatomical_structure Endocrinology Mucociliary Clearance Immunology biology.protein Interleukin-3 Interleukin-4 Bronchial Hyperreactivity business Epithalamus Stress Psychological Respiratory tract |
| Zdroj: | Respiratory Physiology & Neurobiology. 173:79-85 |
| ISSN: | 1569-9048 |
| Popis: | We evaluated if repeated stress modulates mucociliary clearance and inflammatory responses in airways of guinea pigs (GP) with chronic inflammation. The GP received seven exposures of ovalbumin or saline 0.9%. After 4th inhalation, animals were submitted to repeated forced swim stressor protocol (5×/week/2 weeks). After 7th inhalation, GP were anesthetized. We measured transepithelial potential difference, ciliary beat frequency, mucociliary transport, contact angle, cough transportability and serum cortisol levels. Lungs and adrenals were removed, weighed and analyzed by morphometry. Ovalbumin-exposed animals submitted to repeated stress had a reduction in mucociliary transport, and an increase on serum cortisol, adrenals weight, mucus wettability and adhesivity, positive acid mucus area and IL-4 positive cells in airway compared to non-stressed ovalbumin-exposed animals (p < 0.05). There were no effects on eosinophilic recruitment and IL-13 positive cells. Repeated stress reduces mucociliary clearance due to mucus rheological-property alterations, increasing acid mucus and its wettability and adhesivity. These effects seem to be associated with IL-4 activation. © 2010 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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