Isolation and Characterization of the Human and Mouse Homologues (SUPT4H andSupt4h) of the Yeast SPT4 Gene
| Autor: | C. Clare Blackburn, Chun Hui Tsai, Eric Crombez, Richard Im, Pei Wen Chiang, Jason Greenfield, Saravanan Ramamoorthy, Su Qing Wang, David M. Kurnit, Margaret L. Van Keuren, Adnan Akhtar, Woo Joo Song, Paul Smithivas |
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| Rok vydání: | 1996 |
| Předmět: |
Adult
Saccharomyces cerevisiae Proteins Transcription Genetic Pseudogene Genes Fungal Molecular Sequence Data Gene Expression Saccharomyces cerevisiae Biology Polymerase Chain Reaction Conserved sequence Fungal Proteins Mice Fetus Sequence Homology Nucleic Acid Complementary DNA Genetics Animals Humans Coding region Amino Acid Sequence Gene Conserved Sequence DNA Primers Mammals Zinc finger Regulation of gene expression Base Sequence Sequence Homology Amino Acid Chromosome Mapping Nuclear Proteins Zinc Fingers Introns Chromatin DNA-Binding Proteins Repressor Proteins Organ Specificity Protein Biosynthesis Transcriptional Elongation Factors Pseudogenes Chromosomes Human Pair 17 |
| Zdroj: | Genomics. 34:368-375 |
| ISSN: | 0888-7543 |
| Popis: | To study gene regulation mediated by chromatin in mammals, we isolated the human (SUPT4H) and murine (Supt4h) counterparts of the yeast gene encoding SPT4; the product of this gene presumably interacts with the products of the mammalian homologues (which we have also cloned) of yeast SPT5 and SPT6, thereby modulating chromatin formation and activity. We isolated two different sized human SUPT4H cDNA clones (1464 and 728 nt) and one murine Supt4h (688 nt) cDNA clone; all three encode the same 117-amino-acid protein with conservation of the zinc finger motif found in SPT4. Conservation of this zinc finger motif from yeast to mouse and human implies functional importance. Although the overall sequence homology at the DNA level between the human 728-nt transcript and the murine 688-nt transcript is only 78.4%, the DNA sequence homology is 97.7% within the coding region. At the protein level, the amino acid sequences of the translated murine Supt4h and the human SUPT4H gene products are identical. The likely functional copy of SUPT4H, which has at least two introns, maps to human chromosome 17, with candidate intronless pseudogenes on chromosomes 2, 12, and 20. Buttressing the hypothesis that this is a gene required constitutively, both the human SUPT4H transcripts and the murine Supt4h transcript are expressed widely, although not at equal levels (e.g., such as most histones), in all fetal and adult tissues that we examined. |
| Databáze: | OpenAIRE |
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