The heme iron coordination of unfolded ferric and ferrous cytochrome c in neutral and acidic urea solutions. Spectroscopic and electrochemical studies
| Autor: | Milan Fedurco, Jan Augustynski, Chiara Indiani, Giulietta Smulevich, Marián Antalík, Mikuláš Bánó, Erik Sedlák, Mary C. Glascock, John H. Dawson |
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| Rok vydání: | 2004 |
| Předmět: |
Circular dichroism
Protein Denaturation Cytochrome Iron Inorganic chemistry Biophysics Cytochrome c Group Heme Spectrum Analysis Raman Biochemistry Ferric Compounds Analytical Chemistry Ferrous chemistry.chemical_compound medicine Electrochemistry Animals Urea Histidine Ferrous Compounds Horses Molecular Biology biology Cytochrome c peroxidase Cytochrome c Circular Dichroism Water Hydrogen-Ion Concentration Solutions Kinetics chemistry biology.protein Ferric Spectrophotometry Ultraviolet Oxidation-Reduction Nuclear chemistry medicine.drug |
| Zdroj: | Biochimica et biophysica acta. 1703(1) |
| ISSN: | 0006-3002 |
| Popis: | The heme iron coordination of unfolded ferric and ferrous cytochrome c in the presence of 7-9 M urea at different pH values has been probed by several spectroscopic techniques including magnetic and natural circular dichroism (CD), electrochemistry, UV-visible (UV-vis) absorption and resonance Raman (RR). In 7-9 M urea at neutral pH, ferric cytochrome c is found to be predominantly a low spin bis-His-ligated heme center. In acidic 9 M urea solutions the UV-vis and near-infrared (NIR) magnetic circular dichroism (MCD) measurements have for the first time revealed the formation of a high spin His/H(2)O complex. The pK(a) for the neutral to acidic conversion is 5.2. In 9 M urea, ferrous cytochrome c is shown to retain its native ligation structure at pH 7. Formation of a five-coordinate high spin complex in equilibrium with the native form of ferrous cytochrome c takes place below the pK(a) 4.8. The formal redox potential of the His/H(2)O complex of cytochrome c in 9 M urea at pH 3 was estimated to be -0.13 V, ca. 100 mV more positive than E degrees ' estimated for the bis-His complex of cytochrome c in urea solution at pH 7. |
| Databáze: | OpenAIRE |
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