Cytochemical localization and antigenicity of ?-amylase in barley aleurone tissue

Autor:	R. B. Knox, J. V. Jacobsen
Rok vydání:	1973
Předmět:	biology medicine.diagnostic_test Endoplasmic reticulum food and beverages Plant Science Immunofluorescence Aleurone grain membrane Endosperm Biochemistry Cytoplasm Aleurone Genetics biology.protein medicine Amylase Bacterial outer membrane
Zdroj:	Planta. 112:213-224
ISSN:	1432-2048 0032-0935
DOI:	10.1007/bf00385325
Popis:	Gibberellic-acid(GA3)-induced α-amylase has been localised in barley aleurone layers using cytochemical methods and light microscopy. Evidence obtained from the use of a starch substrate film method as well as immunofluorescence indicated that the first amylase to appear in the cell was associated with aleurone grains, apparently with the outer membrane, and also with the peripheral cytoplasm. In GA3-treated tissue, the amylase distribution was much more diffuse, although patchy, throughout the cytoplasm and it tended to accumulate in the endosperm side of the cell. The possibility that the aleurone grain membrane is the site of gibberellin-induced enzyme synthesis and that it proliferates to become rough endoplasmic reticulum is considered. Immunological information was obtained which supports earlier indications that induced α-amylase consists of two different proteins, each with molecular heterogeneity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9e13c894b16e96b08fdc81754c769d5 https://doi.org/10.1007/bf00385325 Zobrazit plný text záznamu Full text from SpringerLink