Prolactin receptors in tilapia (Sarotherodon mossambicus) tissues: binding studies using 125I-labeled ovine prolactin
| Autor: | Marc Edery, Graham Young, Howard A. Bern, Susan Steiny |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Gills
medicine.medical_specialty food.ingredient Receptors Prolactin Urinary Bladder Endogeny Ovary Receptors Cell Surface Biology Kidney Endocrinology food Internal medicine medicine Sarotherodon Animals Intestinal Mucosa Receptor Gonads Sheep Fishes Tilapia biology.organism_classification Prolactin Dissociation constant medicine.anatomical_structure Liver Animal Science and Zoology |
| Zdroj: | General and comparative endocrinology. 56(1) |
| ISSN: | 0016-6480 |
| Popis: | The binding of 125I-labeled ovine prolactin (oPRL) to membrane preparations of tissue from freshwater-adapted tilapia (Sarotherodon mossambicus) was examined. Liver, ovary, and testis showed a relatively high specific binding (5–10%). A lower specific binding occurred consistently in intestine and gill tissue, and inconsistently in urinary bladder and kidney preparations. Desaturation experiments with MgCl2 indicated that a majority of the PRL receptors were already occupied by endogenous PRL. Scatchard analysis of liver binding gave a dissociation constant of 0.6 × 10−9 M and a capacity of 207 fmol/mg protein. |
| Databáze: | OpenAIRE |
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