[Comparative antigen analysis of Trichomonas vaginalis by enzyme-linked immunoelectrotransfer blot technique]
| Autor: | M. H. Leem, Y. K. Choi, Duk-Young Min, Jung Mogg Kim |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
chemistry.chemical_classification
Gel electrophoresis Antiserum Immunoblotting Trichomonas Infections Antigens Protozoan Biology medicine.disease_cause Microbiology Blot Molecular Weight Mice Infectious Diseases Enzyme Immune system chemistry Antigen Homologous antigen medicine Trichomonas vaginalis Animals Parasitology Electrophoresis Polyacrylamide Gel |
| Zdroj: | Kisaengch'unghak chapchi. The Korean journal of parasitology. 30(4) |
| Popis: | Analysis of six isolates of Trichomonas vaginalis was carried out with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunoelectrotransfer blot (EITB). Trichloroacetic acid-treated antigens of the 6 isolates revealed 25 protein profiles ranging 12-170 kDa of molecular weight in SDS-PAGE. In EITB, the specific immunogenic bands were visualized at 51 kDa and 96 kDa when HY-1 antigen was probed with different mice sera immunized with 6 isolates of T. vaginalis. The banding patterns with different sera showed isolate-to-isolate variability. In EITB, homologous antigen (HY-1) did not show any enhanced response in reacting to homologous antiserum (HY-1) when 6 isolates of T. vaginalis were probed with a single serum (HY-1). It is assumed that the different banding patterns of six isolates show isolate-to-isolate variability and immunogenic common bands in 41, 47, 74 and 94 kDa on EITB may connote the important significance on immune response in T. vaginalis infection. |
| Databáze: | OpenAIRE |
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