Production of canine soluble CD40 ligand to induce maturation of monocyte derived dendritic cells for cancer immunotherapy
| Autor: | Shingo Hatoya, Norimitsu Inoue, Daluthgamage Patsy H. Wijesekera, Takashi Akazawa, Kikuya Sugiura, Viskam Wijewardana, Shigeki Imamoto, Toshio Inaba, Mana Yahata |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_treatment
Recombinant Fusion Proteins Immunology CD40 Ligand Molecular Sequence Data chemical and pharmacologic phenomena Cell morphology Monocytes Dogs Cancer immunotherapy Neoplasms medicine Animals Amino Acid Sequence CD86 MHC class II CD40 Expression vector General Veterinary biology Base Sequence Interleukin-12 Subunit p40 hemic and immune systems Immunotherapy Dendritic Cells Molecular biology biology.protein CD80 |
| Zdroj: | Veterinary immunology and immunopathology. 156(1-2) |
| ISSN: | 1873-2534 |
| Popis: | CD40 ligand (CD40L) expressed by activated T cells is shown to induce maturation of immature dendritic cells (DCs) and this maturation is a vital part in DC based tumor immunotherapy. We constructed an expression vector by cloning the extracellular domain of canine CD40L fused to the signal sequence of canine IL-12p40. When PBMCs were incubated with canine granulocyte-macrophage (GM) -CSF and IL-4, expression of CD86 was significantly elevated, but the majority of cells displayed the morphology of immature DCs. Following addition of the expressed canine soluble CD40L (csCD40L) to the DC-inducing culture, the cell morphology shifted to that of mature DCs, and expression of CD80, CD86, MHC class II and CD1a was significantly enhanced. This morphological change and enhancement of expression was observed even when the csCD40L was present only in the second half period of the culture. Furthermore, the csCD40L caused a significant increase in IL-12 production from DCs. These results show that the csCD40L significantly promotes the maturation and activation of canine monocyte derived DCs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect