Tissue-specific expression of the p53 tumor-suppressor gene in the intestine of transgenic mice exposed to DMH and p53 antibodies
| Autor: | P Guervich, T Shkolnik, Y Tendler, Eric Diamond, Oren Zinder, Sophie Lischinsky, I Zusman, B Sandler |
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| Rok vydání: | 1999 |
| Předmět: |
Genetically modified mouse
Cancer Research Ratón Transgene Ileum Mice Transgenic Antibodies Mice Gene expression medicine Animals Humans Genes Tumor Suppressor Intestinal Mucosa Carcinogen Dimethylhydrazines biology General Medicine Molecular biology Intestines medicine.anatomical_structure Oncology Polyclonal antibodies biology.protein Carcinogens Antibody Tumor Suppressor Protein p53 |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1021-335X |
| Popis: | We studied the tissue-specific expression of the p53 gene in different parts of the intestine of mice treated with low doses of a carcinogen and exposed to different p53 antibodies. The human p53 promoter-CAT transgenic mice were immunized with different p53 antibodies (monoclonal-PAb 421 and DOI, and polyclonal-H-p53 and anti-soluble p53 IgG) and then exposed to low doses of dimethyl-hydrazine (DMH). Enzymatic CAT activity was determined in the ileum and colon 8 weeks later after the final injection of DMH. Expression of the p53 transgene in the normal ileum was twice as high as in the colon. Treatment with DMH significantly decreased the expression of the p53 transgene both in the ileum (from 18% to 100%) and in the colon (from 10% to 52%). Vaccination of mice protected at least in part such a decrease. The most effective results were found after exposure of mice to polyclonal H-p53 and to a lesser extent to anti-p53 IgG. No difference was found in the effects of antibodies on the small and large intestines. We concluded that polyclonal antibodies were more effective than monoclonal ones in protection against anti-p53 action of DMH. The observation of these effects may make it possible to explain the higher antitumor activity of poly-clonal to explain the higher antitumor activity of poly-clona antibodies. |
| Databáze: | OpenAIRE |
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