Extramedullary hematopoiesis is dysregulated in histamine-free histidine decarboxylase knockout (HDC−/−) mice
| Autor: | József Fűrész, Ivett Jelinek, Győző Horváth, Zsuzsanna Horváth, Edit I. Buzás, Gizella Veszely, András Falus, Éva Pállinger |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Genetically modified mouse Immunology Spleen Histidine Decarboxylase Biology Flow cytometry Colony-Forming Units Assay Mice chemistry.chemical_compound STAT5 Transcription Factor medicine Animals Regeneration RNA Messenger Cells Cultured Mice Knockout Pharmacology Colony-forming unit Mice Inbred BALB C medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Stem Cells Cell Cycle Flow Cytometry medicine.disease Molecular biology Histidine decarboxylase Extramedullary hematopoiesis Haematopoiesis medicine.anatomical_structure chemistry Hematopoiesis Extramedullary Interleukin-3 Whole-Body Irradiation Histamine |
| Zdroj: | Inflammation Research. 59:429-436 |
| ISSN: | 1420-908X 1023-3830 |
| Popis: | In this study we investigated the role of histamine on the extramedullary hematopoiesis. Male histidine decarboxylase knockout (HDC−/−) mice and wild-type mice were used (n = 5/group). Groups of mice received sublethal total-body gamma irradiation at a single dose of 4 Gy. Spleen cells were studied at different time points post-irradiation by flow cytometry, colony forming unit (CFU) assay, and real-time PCR. For statistical analysis Student’s t test, ANOVA, and Holm-Sidak post-hoc test were used. By day 14 after irradiation, spleen cell counts increased almost eightfold in wild-type and not even fourfold in HDC−/− mice (P |
| Databáze: | OpenAIRE |
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