Development and Quality of Barley Husk Adhesion Correlates With Changes in Caryopsis Cuticle Biosynthesis and Composition
| Autor: | Steve Mitchell, Pete E. Hedley, William T. B. Thomas, Luke Ramsay, Tom Shepherd, Maree Brennan, S. P. Hoad, Jenny Morris, Cairistiona F.E. Topp |
|---|---|
| Přispěvatelé: | Laboratoire d'Etude et de Recherche sur le Matériau Bois (LERMAB), Université de Lorraine (UL), The James Hutton Institute, Scotland's Rural College (SRUC), Department of Cell and Molecular Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Fatty acid elongase complex Plant Science lcsh:Plant culture cementing layer 01 natural sciences Husk grain skinning Caryopsis 03 medical and health sciences [SPI]Engineering Sciences [physics] plant cuticle Lipid biosynthesis Grain quality lcsh:SB1-1110 Food science ComputingMilieux_MISCELLANEOUS Original Research grain development 2. Zero hunger caryopsis husk adhesion Chemistry food and beverages Adhesion barley (Hordeum vulgare) 030104 developmental biology Plant cuticle Composition (visual arts) 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Frontiers, 2019, 10, ⟨10.3389/fpls.2019.00672⟩ Frontiers in Plant Science, Vol 10 (2019) |
| ISSN: | 1664-462X |
| DOI: | 10.3389/fpls.2019.00672⟩ |
| Popis: | The caryopses of barley become firmly adhered to the husk during grain development through a cuticular cementing layer on the caryopsis surface. The degree of this attachment varies among cultivars, with poor quality adhesion causing “skinning”, an economically significant grain quality defect for the malting industry. Malting cultivars encompassing a range of husk adhesion qualities were grown under a misting treatment known to induce skinning. Development of the cementing layer was examined by electron microscopy and compositional changes of the cementing layer were investigated with gas-chromatography followed by mass spectroscopy. Changes in gene expression during adhesion development were examined with a custom barley microarray. The abundance of transcripts involved early in cuticular lipid biosynthesis, including those encoding acetyl-CoA carboxylase, and all four members of the fatty acid elongase complex of enzymes, was significantly higher earlier in caryopsis development than later. Genes associated with subsequent cuticular lipid biosynthetic pathways were also expressed higher early in development, including the decarbonylation and reductive pathways, and sterol biosynthesis. Changes in cuticular composition indicate that lowered proportions of alkanes and higher proportions of fatty acids are associated with development of good quality husk adhesion, in addition to higher proportions of sterols. |
| Databáze: | OpenAIRE |
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